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Isolation and characterization of microsatellites in Portunus trituberculatus 期刊论文
CONSERVATION GENETICS RESOURCES, 2012, 卷号: 4, 期号: 2, 页码: 251-255
作者:  Cui, Zhaoxia;  Liu, Yuan;  Wang, Hongxia;  Wu, Danhua;  Luan, Weisha;  Tan, Feng;  Huang, Minde;  Cui, ZX (reprint author), Chinese Acad Sci, EMBL, Inst Oceanol, Qingdao 266071, Peoples R China.
Adobe PDF(190Kb)  |  收藏  |  浏览/下载:329/0  |  提交时间:2013/09/24
Portunus Trituberculatus  Microsatellites  Polymorphism  
一种海蜇基因组DNA的提取方法-1 专利
专利类型: 发明, 专利号: CN201010166122.X, 申请日期: 2011-03-23, 公开日期: 2011-03-23
发明人:  崔朝霞;  张姝;  栾维莎;  刘媛
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一种海蜇基因组dna提取方法  其特征在于:1)将海蜇有性世代的海蜇碟状体置入体积浓度为95%的酒精中脱水并保存  2)将保存的蝶状体样品放入离心管中并直接加入buffer300~400ul匀浆、30~40ul质量浓度为10%的sds、3~5ul浓度为20mg/ml的蛋白酶k  封口后55~56℃消化0.8~1.0小时  3)将步骤2)中消化后的溶液取出加入6m?nacl  待用  振荡浑匀20~30s  10000~12000rpm离心20?30分钟  吸上清  而后加入上清液的0.7~1倍体积的异丙醇  ?20~?18℃沉淀10~15分钟  沉淀后取出  以10000~12000rpm离心15?20分钟  弃去液体  并用体积浓度为70%的乙醇  离心洗1~2次  自然室温凉干  即得到海蜇碟状体的dna。  
Three isoforms of anti-lipopolysaccharide factor identified from eyestalk cDNA library of swimming crab Portunus trituberculatus 期刊论文
FISH & SHELLFISH IMMUNOLOGY, 2011, 卷号: 30, 期号: 2, 页码: 583-591
作者:  Liu, Yuan;  Cui, Zhaoxia;  Luan, Weisha;  Song, Chengwen;  Nie, Qing;  Wang, Shuangyan;  Li, Qianqian
Adobe PDF(1908Kb)  |  收藏  |  浏览/下载:571/2  |  提交时间:2012/07/03
Portunus Trituberculatus  Anti-lipopolysaccharide Factor  Snp  Expression Analysis  
Molecular cloning and characterization of a serine proteinase homolog prophenoloxidase-activating factor in the swimming crab Portunus trituberculatus 期刊论文
FISH & SHELLFISH IMMUNOLOGY, 2010, 卷号: 29, 期号: 4, 页码: 679-686
作者:  Cui, Zhaoxia;  Liu, Yuan;  Wu, Danhua;  Luan, Weisha;  Wang, Shuangyan;  Li, Qianqian;  Song, Chengwen
Adobe PDF(1536Kb)  |  收藏  |  浏览/下载:355/0  |  提交时间:2010/12/24
Portunus Trituberculatus  Serine Protein Homologous  Eyestalk  Haemocyte  
一种三疣梭子蟹快速生长品系的构建方法 专利
专利类型: 发明, 专利号: CN200810160014.4, 申请日期: 2010-06-16, 公开日期: 2010-06-16
发明人:  崔朝霞;  谭烽;  王春琳;  吴丹华;  栾维莎
Adobe PDF(438Kb)  |  收藏  |  浏览/下载:186/0  |  提交时间:2014/08/04
一种三疣梭子蟹快速生长品系的构建方法  其特征在于:利用选育材料的基因组微卫星作为分子标记  根据分子标记得到遗传结构中存在生殖隔离明显的群体作为杂交群体  杂交群体进行杂交组合  即得三疣梭子蟹快速生长品系。  
Molecular cloning and characterization of a heat shock protein 70 gene in swimming crab (Portunus trituberculatus) 期刊论文
FISH & SHELLFISH IMMUNOLOGY, 2010, 卷号: 28, 期号: 1, 页码: 56-64
作者:  Cui, Zhaoxia;  Liu, Yuan;  Luan, Weisha;  Li, Qianqian;  Wu, Danhua;  Wang, Shuangyan
Adobe PDF(1994Kb)  |  收藏  |  浏览/下载:343/0  |  提交时间:2015/07/20
Portunus Trituberculatus  Heat Shock Protein 70  Snp  Mrna Expression  Vibrio Alginolyticus  
Molecular identification of Pampus fishes (Perciformes, Stromateidae) 期刊论文
ICHTHYOLOGICAL RESEARCH, 2010, 卷号: 57, 期号: 1, 页码: 32-39
作者:  Cui, Zhaoxia;  Liu, Yuan;  Liu, Jing;  Luan, Weisha
Adobe PDF(289Kb)  |  收藏  |  浏览/下载:212/3  |  提交时间:2011/07/25
Pampus  Cytochrome c Oxidase i  16s Rrna  Pampus Minor  
Permanent Genetic Resources added to Molecular Ecology Resources Database 1 May 2009-31 July 2009 期刊论文
MOLECULAR ECOLOGY RESOURCES, 2009, 卷号: 9, 期号: 6, 页码: 1460-1466
作者:  Almany, Glenn R.;  De Arruda, Mauricio P.;  Arthofer, Wolfgang;  Atallah, Z. K.;  Beissinger, Steven R.;  Berumen, Michael L.;  Bogdanowicz, S. M.;  Brown, S. D.;  Bruford, Michael W.;  Burdine, C.;  Busch, Jeremiah W.;  Campbell, Nathan R.;  Carey, D.;  Carstens, Bryan C.;  Chu, K. H.;  Cubeta, Marc A.;  Cuda, J. P.;  Cui, Zhaoxia;  Datnoff, L. E.;  Davila, J. A.;  Davis, Emily S.;  Davis, R. M.;  Diekmann, Onno E.;  Eizirik, Eduardo;  Fargallo, J. A.;  Fernandes, Fabiano;  Fukuda, Hideo;  Gale, L. R.;  Gallagher, Elizabeth;  Gao, Yongqiang;  Girard, Philippe;  Godhe, Anna;  Goncalves, Evonnildo C.;  Gouveia, Licinia;  Grajczyk, Amber M.;  Grose, M. J.;  Gu, Zhifeng;  Hallden, Christer;  Harnstrom, Karolina;  Hemmingsen, Amanda H.;  Holmes, Gerald;  Huang, C. H.;  Huang, Chuan-Chin;  Hudman, S. P.;  Jones, Geoffrey P.;  Kanetis, Loukas;  Karunasagar, Iddya;  Karunasagar, Indrani;  Keyghobadi, Nusha;  Klosterman, S. J.;  Klug, Page E.;  Koch, J.;  Koopman, Margaret M.;  Koeppler, Kirsten;  Koshimizu, Eriko;  Krumboeck, Susanne;  Kubisiak, T.;  Landis, J. B.;  Lasta, Mario L.;  Lee, Chow-Yang;  Li, Qianqian;  Li, Shou-Hsien;  Lin, Rong-Chien;  Liu, M.;  Liu, Na;  Liu, W. C.;  Liu, Yuan;  Loiseau, A.;  Luan, Weisha;  Maruthachalam, K. K.;  McCormick, Helen M.;  Mellick, Rohan;  Monnahan, P. J.;  Morielle-Versute, Eliana;  Murray, Tomas E.;  Narum, Shawn R.;  Neufeld, Katie;  de Nova, P. J. G.;  Ojiambo, Peter S.;  Okamoto, Nobuaki;  Othman, Ahmad Sofiman;  Overholt, W. A.;  Pardini, Renata;  Paterson, Ian G.;  Patty, Olivia A.;  Paxton, Robert J.;  Planes, Serge;  Porter, Carolyn;  Pratchett, Morgan S.;  Puettker, Thomas;  Rasic, Gordana;  Rasool, Bilal;  Rey, O.;  Riegler, Markus;  Riehl, C.;  Roberts, John M. K.;  Roberts, P. D.;  Rochel, Elisabeth;  Roe, Kevin J.;  Rossetto, Maurizio;  Ruzzante, Daniel E.;  Sakamoto, Takashi;  Saravanan, V.;  Sarturi, Cladinara Roberts;  Schmidt, Anke;  Cruz Schneider, Maria Paula;  Schuler, Hannes;  Serb, Jeanne M.;  Serrao, Ester T. A.;  Shi, Yaohua;  Silva, Artur;  Sin, Y. W.;  Sommer, Simone;  Stauffer, Christian;  Struessmann, Carlos Augusto;  Subbarao, K. V.;  Syms, Craig;  Tan, Feng;  Tejedor, Eugenio Daniel;  Thorrold, Simon R.;  Trigiano, Robert N.;  Trucco, Maria I.;  Nunes Tsuchiya-Jerep, Mirian Tieko;  Vergara, P.;  van de Vliet, Mirjam S.;  Wadl, Phillip A.;  Wang, Aimin;  Wang, Hongxia;  Wang, R. X.;  Wang, Xinwang;  Wang, Yan;  Weeks, Andrew R.;  Wei, Fuwen;  Werner, William J.;  Wiley, E. O.;  Williams, D. A.;  Wilkins, Richard J.;  Wisely, Samantha M.;  With, Kimberly A.;  Wu, Danhua;  Yao, Cheng-Te;  Yau, Cynthia;  Yeap, Beng-Keok;  Zhai, Bao-Ping;  Zhan, Xiangjiang;  Zhang, Guo-Yan;  Zhang, S. Y.;  Zhao, Ru;  Zhu, Lifeng;  Mol Ecol Resources;  Almany, GR, James Cook Univ, ARC Ctr Excellence Coral Reef Studies, Townsville, Qld 4811, Australia
Adobe PDF(121Kb)  |  收藏  |  浏览/下载:607/0  |  提交时间:2010/11/18
一种海蜇基因组DNA的提取方法 专利
专利类型: 发明, 专利号: CN200710015295.X, 申请日期: 2009-01-14, 公开日期: 2009-01-14
发明人:  崔朝霞;  张姝;  栾维莎;  刘媛
Adobe PDF(376Kb)  |  收藏  |  浏览/下载:365/2  |  提交时间:2014/08/04
1.一种海蛰基因组dna提取方法  其特征在于:1)将海蜇无性世代的海蛰螅状体用手术剪刀剪碎至无明显颗粒状组织  2)将步骤1)得到的原料内加入3~5ul浓度为20mg/ml的蛋白酶k  加入ctab Buffer300~400ul  在55~56℃消化24~26小时至溶液澄清  3)在步骤2)澄清溶液中加入与其等体积的苯酚、氯仿和异戊醇的混合溶液混匀  待用  待用  离心  而后向上清液中加入上清液的0.7~1倍体积的异丙醇  吸上清  -20~-18℃沉淀10~15分钟  其中苯酚、氯仿和异戊醇按体积份数比为25∶24∶1  再加入与上清液等体积的氯仿和异戊醇混合液混匀  沉淀后取出  氯仿和异戊醇按体积份数比为24∶1。  离心  离心  吸上清  弃去液体  并用体积浓度为70%的乙醇  离心洗1~2次  将多余液体倒去室温自然凉干  即得到海蛰螅状体的基因组dna