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| Three novel B-type mannose-specific lectins of Cynoglossus semilaevis possess varied antibacterial activities against Gram-negative and Gram-positive bacteria | |
Sun, Yuan-yuan1,2,4 ; Liu, Li1,2,4; Li, Jun3; Sun, Li1,2
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| 2016-02-01 | |
| 发表期刊 | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
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| 卷号 | 55期号:2016页码:194-202 |
| 文章类型 | Article |
| 摘要 | Lectins are a group of sugar-binding proteins that are important factors of the innate immune system. In this study, we examined, in a comparative manner, the expression and function of three Bulb-type (B-type) mannose-specific lectins (named CsBML1, CsBML2, and CsBML3) from tongue sole. All three lectins possess three repeats of the conserved mannose binding motif QXDXNXVX'Y. Expression of CsBML1, CsBML2, and CsBML3 was most abundant in liver and upregulated by bacterial infection. Recombinant (r) CsBML1, CsBML2, and CsBML3 bound to a wide arrange of bacteria in a dose-dependent manner and with different affinities. All three lectins displayed mannose-specific and calcium-dependent agglutinating capacities but differed in agglutinating profiles. rCsBML1 and rCsBML2, but not rCsBML3, killed target bacteria in vitro and inhibited bacterial dissemination in fish tissues in vivo. These results indicate for the first time that in teleost, different members of B-type mannose-specific lectins likely play different roles in antibacterial immunity. (C) 2015 Elsevier Ltd. All rights reserved. |
| 关键词 | B-type Lectin Cynoglossus Semilaevis Agglutination Antibacterial |
| DOI | 10.1016/j.dci.2015.10.003 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000368382200022 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/95218 |
| 专题 | 实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 3.Lake Super State Univ, Sch Biol Sci, Sault Ste Marie, MI USA 4.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 第一作者单位 | 实验海洋生物学重点实验室 |
| 推荐引用方式 GB/T 7714 | Sun, Yuan-yuan,Liu, Li,Li, Jun,et al. Three novel B-type mannose-specific lectins of Cynoglossus semilaevis possess varied antibacterial activities against Gram-negative and Gram-positive bacteria[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2016,55(2016):194-202. |
| APA | Sun, Yuan-yuan,Liu, Li,Li, Jun,&Sun, Li.(2016).Three novel B-type mannose-specific lectins of Cynoglossus semilaevis possess varied antibacterial activities against Gram-negative and Gram-positive bacteria.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,55(2016),194-202. |
| MLA | Sun, Yuan-yuan,et al."Three novel B-type mannose-specific lectins of Cynoglossus semilaevis possess varied antibacterial activities against Gram-negative and Gram-positive bacteria".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 55.2016(2016):194-202. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Three novel B-type m（2833KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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