Genetic analysis of meio- and mito-gynogenetic stocks of Paralichthys olivaceus with microsatellite markers

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	Genetic analysis of meio- and mito-gynogenetic stocks of Paralichthys olivaceus with microsatellite markers
	Wang Wei 1,2; You Feng 1; Xu Jianhe 1; Sun Wei 1; Zhu Xiangping 1; Gao Tianxiang 2; Zhang Peijun 1
	2008
发表期刊	ACTA OCEANOLOGICA SINICA
ISSN	0253-505X
卷号	27 期号:2 页码:149-156
文章类型	Article
摘要	Twenty microsatellite markers (Po1, Po13, Po33, Po35, Po42, Po48, Po56, Po89, Po91, kop6, kop7, kop8, kop12, kop15, kop18, kop21, kop22, kop23, kop26, Po-strl) were used to assess the meiogynogenetic and mitogynogenetic stocks of the left-eyed flounder, Paralichthys olivaceus, which were derived from single pair crossing. Twelve of the 20 loci utilized showed heterozygosity in the female and were mapped in relation to their centromeres in the meiogynogenetic diploid flounder. Microsatellite-centromere map distance, calculated under the assumption of complete interference, ranged from 15.8 cM for kop22 to 50 cM for Po13, Po56 and Po89. Excluding the kop22, the heterozygosities of the rest of the loci were close to 100%, suggesting the occurrence of near complete interference on the chromosome arms that carried these loci. In the mitogynogenetic diploid flounder, each individual showed exact homozygousity and the segregation profiles did not deviate from the Mendelian 1: 1 pattern. The results indicated that there was no lethal gene linked with the loci analyzed. Such high interference accounted for the high recombination rates and large map distances. The Po13 and Po56 loci, Po91 and kop18 loci, kop15 and kop21 loci are tightly linked on the same chromosome arm in pairs.; Twenty microsatellite markers (Po1, Po13, Po33, Po35, Po42, Po48, Po56, Po89, Po91, kop6, kop7, kop8, kop12, kop15, kop18, kop21, kop22, kop23, kop26, Po-strl) were used to assess the meiogynogenetic and mitogynogenetic stocks of the left-eyed flounder, Paralichthys olivaceus, which were derived from single pair crossing. Twelve of the 20 loci utilized showed heterozygosity in the female and were mapped in relation to their centromeres in the meiogynogenetic diploid flounder. Microsatellite-centromere map distance, calculated under the assumption of complete interference, ranged from 15.8 cM for kop22 to 50 cM for Po13, Po56 and Po89. Excluding the kop22, the heterozygosities of the rest of the loci were close to 100%, suggesting the occurrence of near complete interference on the chromosome arms that carried these loci. In the mitogynogenetic diploid flounder, each individual showed exact homozygousity and the segregation profiles did not deviate from the Mendelian 1: 1 pattern. The results indicated that there was no lethal gene linked with the loci analyzed. Such high interference accounted for the high recombination rates and large map distances. The Po13 and Po56 loci, Po91 and kop18 loci, kop15 and kop21 loci are tightly linked on the same chromosome arm in pairs.
关键词	Paralichthys Olivaceus Meiogynogenesis Mitogynogenesis Microsatellite-centromere Mapping
收录类别	SCI
语种	英语
WOS记录号	WOS:000256131900013
引用统计	被引频次：5[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/6298
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Ocean Univ China, Coll Life Sci & Technol, Qingdao 266003, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Wang Wei,You Feng,Xu Jianhe,et al. Genetic analysis of meio- and mito-gynogenetic stocks of Paralichthys olivaceus with microsatellite markers[J]. ACTA OCEANOLOGICA SINICA,2008,27(2):149-156.
APA	Wang Wei.,You Feng.,Xu Jianhe.,Sun Wei.,Zhu Xiangping.,...&Zhang Peijun.(2008).Genetic analysis of meio- and mito-gynogenetic stocks of Paralichthys olivaceus with microsatellite markers.ACTA OCEANOLOGICA SINICA,27(2),149-156.
MLA	Wang Wei,et al."Genetic analysis of meio- and mito-gynogenetic stocks of Paralichthys olivaceus with microsatellite markers".ACTA OCEANOLOGICA SINICA 27.2(2008):149-156.

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