Molecular cloning and expression analysis of Crustin-like gene from Chinese shrimp Fenneropenaeus chinensis

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	Molecular cloning and expression analysis of Crustin-like gene from Chinese shrimp Fenneropenaeus chinensis
	Liu Fengsong 1,2; Li Fuhua 1; Xiang Jianhai1 ; Dong Bo 1; Liu Yichen 1; Zhang Xiaojun 1; Zhang Liusuo 1
	2008
发表期刊	ACTA OCEANOLOGICA SINICA
ISSN	0253-505X
卷号	27 期号:2 页码:81-92
文章类型	Article
摘要	A new member of antimicrobial protein genes of the Crustin family was cloned from haemocytes of the Chinese shrimp Fenneropenaeus chinensis by 3' and 5' RACE. The full-length cDNA of Crustin-like gene contains a 390 bp open reading frame, encoding 130 amino acids. The deduced peptide contains a putative signal peptide of 17 amino acids and mature peptide of 113 amino acids. The molecular mass of the deduced mature peptide is 12.3 ku. It is highly cationic with a theoretical isoelectric point of 8.5. The deduced amino acids sequence of this Crustin showed high homology with those of Penaeus (Litopenaeas) setferus. Northern blotting showed that the cloned Crustin gene was mainly expressed in haemocytes, gill, intestine, and RNA in situ hybridization indicated that the Crustin gene was constitutively expressed exclusively in haemocytes of these tissues. Capillary electrophoresis RT-PCR analysis showed that Crustin was up-regulated dramatically from 12 to 48 h after a brief decrease of mRNA during first 6 h in response to microbe infection. The level of Crustin mRNA began to restore at 72 h post-challenge. This indicated that Crustin gene might play an important role when shrimps are infected by bacterial pathogen.; A new member of antimicrobial protein genes of the Crustin family was cloned from haemocytes of the Chinese shrimp Fenneropenaeus chinensis by 3' and 5' RACE. The full-length cDNA of Crustin-like gene contains a 390 bp open reading frame, encoding 130 amino acids. The deduced peptide contains a putative signal peptide of 17 amino acids and mature peptide of 113 amino acids. The molecular mass of the deduced mature peptide is 12.3 ku. It is highly cationic with a theoretical isoelectric point of 8.5. The deduced amino acids sequence of this Crustin showed high homology with those of Penaeus (Litopenaeas) setferus. Northern blotting showed that the cloned Crustin gene was mainly expressed in haemocytes, gill, intestine, and RNA in situ hybridization indicated that the Crustin gene was constitutively expressed exclusively in haemocytes of these tissues. Capillary electrophoresis RT-PCR analysis showed that Crustin was up-regulated dramatically from 12 to 48 h after a brief decrease of mRNA during first 6 h in response to microbe infection. The level of Crustin mRNA began to restore at 72 h post-challenge. This indicated that Crustin gene might play an important role when shrimps are infected by bacterial pathogen.
关键词	Crustin-like Gene Fenaeropenaeius Chinensis Molecular Cloning Expression Analysis Microbe Challenge
收录类别	SCI
语种	英语
WOS记录号	WOS:000256131900007
引用统计	被引频次：8[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/6292
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Expt Marine Biol Lab, Qingdao 266071, Peoples R China 2.Hebei Univ, Coll Life Sci, Baoding 071002, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Liu Fengsong,Li Fuhua,Xiang Jianhai,et al. Molecular cloning and expression analysis of Crustin-like gene from Chinese shrimp Fenneropenaeus chinensis[J]. ACTA OCEANOLOGICA SINICA,2008,27(2):81-92.
APA	Liu Fengsong.,Li Fuhua.,Xiang Jianhai.,Dong Bo.,Liu Yichen.,...&Zhang Liusuo.(2008).Molecular cloning and expression analysis of Crustin-like gene from Chinese shrimp Fenneropenaeus chinensis.ACTA OCEANOLOGICA SINICA,27(2),81-92.
MLA	Liu Fengsong,et al."Molecular cloning and expression analysis of Crustin-like gene from Chinese shrimp Fenneropenaeus chinensis".ACTA OCEANOLOGICA SINICA 27.2(2008):81-92.

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