Determination of tetrodotoxin by liquid chromatography coupled with electrospray ion-trap mass spectrometry

	Determination of tetrodotoxin by liquid chromatography coupled with electrospray ion-trap mass spectrometry
	Li Ai-Feng ; Yu Ren-Cheng ; Zhou Ming-Jiang
	2007-03-01
发表期刊	CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
ISSN	0253-3820
卷号	35 期号:3 页码:397-400
文章类型	Article
摘要	Two methods for tetrodotoxin analysis using liquid chromatography coupled with electrospray iontrap mass spectrometry (LC-ESI-MS) have been established with C,, reversed phase column and hydrophilic interaction liquid chromatography (HILIC) column, respectively. Sensitivity and reproducibility of the methods were compared. The method using C-18 column in selected ion monitoring (SIM) mode had a detection limit (S/N = 3) of 120 pg, and a good linearity of the calibration curve was obtained for tetrodotoxin (r = 0. 9992). High reproducibility of the method was observed, with a relative standard deviation (RSD) below 10%. The method using HILIC column in SIM mode and selected reaction monitoring (SRM) mode had detection limits (S/N = 3) of 15 and 3.75 pg, respectively. Good linearity of the calibration curves was obtained for tetrodotoxin (r = 0. 9996 and 0. 9998 in SIM and SRM mode, respectively). T he reproducibility was high in SIM mode but relatively poor in SRM mode. Based on the results, the method using HILIC column in SIM mode was suggested for the analysis of tetrodotoxin with LC-MS system.; Two methods for tetrodotoxin analysis using liquid chromatography coupled with electrospray iontrap mass spectrometry (LC-ESI-MS) have been established with C,, reversed phase column and hydrophilic interaction liquid chromatography (HILIC) column, respectively. Sensitivity and reproducibility of the methods were compared. The method using C-18 column in selected ion monitoring (SIM) mode had a detection limit (S/N = 3) of 120 pg, and a good linearity of the calibration curve was obtained for tetrodotoxin (r = 0. 9992). High reproducibility of the method was observed, with a relative standard deviation (RSD) below 10%. The method using HILIC column in SIM mode and selected reaction monitoring (SRM) mode had detection limits (S/N = 3) of 15 and 3.75 pg, respectively. Good linearity of the calibration curves was obtained for tetrodotoxin (r = 0. 9996 and 0. 9998 in SIM and SRM mode, respectively). T he reproducibility was high in SIM mode but relatively poor in SRM mode. Based on the results, the method using HILIC column in SIM mode was suggested for the analysis of tetrodotoxin with LC-MS system.
关键词	Liquid Chromatography With Electrospray Ion-tap Mass Spectrometry Tetrodotoxin Selected Ion Monitoring Selected Reaction Monitoring
收录类别	SCI
语种	英语
WOS记录号	WOS:000245735200018
引用统计	被引频次：8[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/6260
专题	海洋生态与环境科学重点实验室
作者单位	1.Ocean Univ China, Key Lab Marine Environm Sci & Ecol, Qingdao 266100, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Li Ai-Feng,Yu Ren-Cheng,Zhou Ming-Jiang. Determination of tetrodotoxin by liquid chromatography coupled with electrospray ion-trap mass spectrometry[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2007,35(3):397-400.
APA	Li Ai-Feng,Yu Ren-Cheng,&Zhou Ming-Jiang.(2007).Determination of tetrodotoxin by liquid chromatography coupled with electrospray ion-trap mass spectrometry.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,35(3),397-400.
MLA	Li Ai-Feng,et al."Determination of tetrodotoxin by liquid chromatography coupled with electrospray ion-trap mass spectrometry".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 35.3(2007):397-400.

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