| A preliminary genetic linkage map of the pacific abalone Haliotis discus hannai Ino | |
| Liu, Xiande; Liu, Xiao; Guo, Ximing; Gao, Qikang; Zhao, Hongen; Zhang, Guofan | |
| 2006-07-01 | |
| 发表期刊 | MARINE BIOTECHNOLOGY
 |
| ISSN | 1436-2228 |
| 卷号 | 8期号:4页码:386-397 |
| 文章类型 | Article |
| 摘要 | Preliminary genetic linkage maps were constructed for the Pacific abalone (Haliotis discus hannai Ino) using amplified fragment length polymorphism (AFLP), randomly amplified polymorphic DNA (RAPD), and microsatellite markers segregating in a F, family. Nine microsatellite loci, 41 RAPD, and 2688 AFLP markers were genotyped in the parents and 86 progeny of the mapping family. Among the 2738 markers, 384 (including 365 AFLP markers, 10 RAPD markers, and 9 microsatellite loci) were polymorphic and segregated in one or both parents: 241 in the female and 146 in the male. The majority of these markers, 232 in the female and 134 in the male, segregated according to the expected 1:1 Mendelian ratio (alpha = 0.05). Two genetic linkage maps were constructed using markers segregating in the female or the male parent. The female framework map consisted of 119 markers in 22 linkage groups, covering 1773.6 cM with an average intermarker space of 18.3 cM. The male framework map contained 94 markers in 19 linkage groups, spanning 1365.9 cM with an average intermarker space of 18.2 cM. The sex determination locus was mapped to the male map but not to the female map, suggesting a XY-male determination mechanism. Distorted markers showing excess of homozygotes were mapped in clusters, probably because of their linkage to a gene that is incompatible between two parental populations.; Preliminary genetic linkage maps were constructed for the Pacific abalone (Haliotis discus hannai Ino) using amplified fragment length polymorphism (AFLP), randomly amplified polymorphic DNA (RAPD), and microsatellite markers segregating in a F, family. Nine microsatellite loci, 41 RAPD, and 2688 AFLP markers were genotyped in the parents and 86 progeny of the mapping family. Among the 2738 markers, 384 (including 365 AFLP markers, 10 RAPD markers, and 9 microsatellite loci) were polymorphic and segregated in one or both parents: 241 in the female and 146 in the male. The majority of these markers, 232 in the female and 134 in the male, segregated according to the expected 1:1 Mendelian ratio (alpha = 0.05). Two genetic linkage maps were constructed using markers segregating in the female or the male parent. The female framework map consisted of 119 markers in 22 linkage groups, covering 1773.6 cM with an average intermarker space of 18.3 cM. The male framework map contained 94 markers in 19 linkage groups, spanning 1365.9 cM with an average intermarker space of 18.2 cM. The sex determination locus was mapped to the male map but not to the female map, suggesting a XY-male determination mechanism. Distorted markers showing excess of homozygotes were mapped in clusters, probably because of their linkage to a gene that is incompatible between two parental populations. |
| 关键词 | Amplified Fragment Length Polymorphism (Aflp) Genetic Linkage Map Haliotis Discus Hannai Ino Microsatellite Randomly Amplified Polymorphic Dna (Rapd) Sex Determination |
| 学科领域 | Biotechnology & Applied Microbiology ; Marine & Freshwater Biology |
| DOI | 10.1007/s10126-005-6133-7 |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000239656600008 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/6146 |
| 专题 | 海洋生物技术研发中心 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Grad Univ, Chinese Acad Sci, Beijing 100049, Peoples R China 3.Rutgers State Univ, Inst Marine & Coastal Sci, Haskin Shellfish Res Lab, Port Norris, NJ 08349 USA 4.Zhejiang Univ, Hangzhou 310029, Zhejiang, Peoples R China 5.Dalian Inst Fisheries, Dalian 116013, Liaonin, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Xiande,Liu, Xiao,Guo, Ximing,et al. A preliminary genetic linkage map of the pacific abalone Haliotis discus hannai Ino[J]. MARINE BIOTECHNOLOGY,2006,8(4):386-397. |
| APA | Liu, Xiande,Liu, Xiao,Guo, Ximing,Gao, Qikang,Zhao, Hongen,&Zhang, Guofan.(2006).A preliminary genetic linkage map of the pacific abalone Haliotis discus hannai Ino.MARINE BIOTECHNOLOGY,8(4),386-397. |
| MLA | Liu, Xiande,et al."A preliminary genetic linkage map of the pacific abalone Haliotis discus hannai Ino".MARINE BIOTECHNOLOGY 8.4(2006):386-397. |
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