| Factors influencing hemolytic activity of venom from the jellyfish Rhopilema esculentum Kishinouye | |
Yu, Huahua ; Li, Cuiping; Li, Ronggui; Xing, Ronge; Liu, Song; Li, Pengcheng
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| 2007-07-01 | |
| 发表期刊 | FOOD AND CHEMICAL TOXICOLOGY
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| ISSN | 0278-6915 |
| 卷号 | 45期号:7页码:1173-1178 |
| 文章类型 | Article |
| 摘要 | In this study, hemolytic activity of venom from the jellyfish Rhopilema esculentum Kishinouye and some factors affecting it were assayed. The HU(50) of R. esculentum full venom (RFV) against chicken erythrocytes was 3.40 mu g/ml and a Hill coefficient value was 1.73 suggesting at least two molecules participated in hemolytic activity. The hemolytic activity of RFV was affected by some chemical and physical factors such as divalent cations, EDTA, (NH(4))(2)SO(4), pH and temperature. In the presence of Mg(2+), Cu(2+), Zn(2+), Fe(2+), Ca(2+) ( >= 2 mM), Mn(2+) (>= 1 mM), EDTA (>= 2 mM) and (NH(4))(2)SO(4), the hemolytic activity of RFV was reduced. RFV had strong hemolytic activity at the pH 6-10 and the hemolytic ratios were 0.95-1.19. Hemolytic activity was temperature-sensitive and when RFV was pre-incubated at temperatures over 40 degrees C, it was sharply reduced. (c) 2007 Elsevier Ltd. All rights reserved.; In this study, hemolytic activity of venom from the jellyfish Rhopilema esculentum Kishinouye and some factors affecting it were assayed. The HU50 of R. esculentum full venom (RFV) against chicken erythrocytes was 3.40 mu g/ml and a Hill coefficient value was 1.73 suggesting at least two molecules participated in hemolytic activity. The hemolytic activity of RFV was affected by some chemical and physical factors such as divalent cations, EDTA, (NH4)(2)SO4, pH and temperature. In the presence of Mg2+, Cu2+, Zn2+, Fe2+, Ca2+ ( >= 2 mM), Mn2+ (>= 1 mM), EDTA (>= 2 mM) and (NH4)(2)SO4, the hemolytic activity of RFV was reduced. RFV had strong hemolytic activity at the pH 6-10 and the hemolytic ratios were 0.95-1.19. Hemolytic activity was temperature-sensitive and when RFV was pre-incubated at temperatures over 40 degrees C, it was sharply reduced. (c) 2007 Elsevier Ltd. All rights reserved. |
| 关键词 | Hemolytic Activity Jellyfish Rhopilema Esculentum Kishinouye Venom |
| 学科领域 | Food Science & Technology ; Toxicology |
| DOI | 10.1016/j.fct.2006.12.025 |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000247537800011 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/6112 |
| 专题 | 海洋生物技术研发中心 海洋环境工程技术研究发展中心 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yu, Huahua,Li, Cuiping,Li, Ronggui,et al. Factors influencing hemolytic activity of venom from the jellyfish Rhopilema esculentum Kishinouye[J]. FOOD AND CHEMICAL TOXICOLOGY,2007,45(7):1173-1178. |
| APA | Yu, Huahua,Li, Cuiping,Li, Ronggui,Xing, Ronge,Liu, Song,&Li, Pengcheng.(2007).Factors influencing hemolytic activity of venom from the jellyfish Rhopilema esculentum Kishinouye.FOOD AND CHEMICAL TOXICOLOGY,45(7),1173-1178. |
| MLA | Yu, Huahua,et al."Factors influencing hemolytic activity of venom from the jellyfish Rhopilema esculentum Kishinouye".FOOD AND CHEMICAL TOXICOLOGY 45.7(2007):1173-1178. |
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