| Strain H-2-419-4 of Haematococcus pluvialis induced by ethyl methanesulphonate and ultraviolet radiation | |
| Sun Yanhong; Liu Jianguo; Zhang Xiaoli; Lin Wei | |
| 2008 | |
| 发表期刊 | CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
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| ISSN | 0254-4059 |
| 卷号 | 26期号:2页码:152-156 |
| 摘要 | Two strains H-2-410 and H-2-419 were obtained from the chemically mutated survivors of wild Haematococcus pluvialis 2 by using ethyl methanesulphonate (EMS). Strains H2-410 and H2-419 showed a fast cell growth with 13% and 20% increase in biomass compared to wild type, respectively. Then H-2-419-4, a fast cell growth and high astaxanthin accumulation strain, was obtained by exposing the strain H2-419 to ultraviolet radiation (UV) further. The total biomass, the astaxanthin content per cell, astaxanthin production of H-2-4194 showed 68%, 28%, and 120% increase compared to wild H. pluvialis 2, respectively. HPLC (High Performance Liquid Chromatography) data showed also an obvious proportional variation of different carotenoid compositions in the extracts of H2-4194 and the wild type, although no peak of carotenoids appeared or disappeared. Therefore, the main compositions in strain H-2-419-4, like its wild one, were free of astaxanthin, monoester, and diester of astaxanthin. The asexual reproduction in survivors after exposed to UV was not synchronous, and different from the normal synchronous asexual reproduction as the mother cells were motile instead of non-motile. Interestingly, some survivors from UV irradiation produced many mini-spores (or gamete?), the spores moved away from the mother cell gradually 4 or 5 days later. This is quite similar to sexual reproduction described by Elliot in 1934. However, whether this was sexual reproduction remains questionable, as no mating process has been observed. |
| 关键词 | Astaxanthin Accumulation |
| 学科领域 | Limnology ; Oceanography |
| URL | 查看原文 |
| 收录类别 | sci |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/6094 |
| 专题 | 海洋生物技术研发中心 |
| 推荐引用方式 GB/T 7714 | Sun Yanhong,Liu Jianguo,Zhang Xiaoli,et al. Strain H-2-419-4 of Haematococcus pluvialis induced by ethyl methanesulphonate and ultraviolet radiation[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2008,26(2):152-156. |
| APA | Sun Yanhong,Liu Jianguo,Zhang Xiaoli,&Lin Wei.(2008).Strain H-2-419-4 of Haematococcus pluvialis induced by ethyl methanesulphonate and ultraviolet radiation.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,26(2),152-156. |
| MLA | Sun Yanhong,et al."Strain H-2-419-4 of Haematococcus pluvialis induced by ethyl methanesulphonate and ultraviolet radiation".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 26.2(2008):152-156. |
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