Effect of cryoprotectants on hatching rate of red seabream (Pagrus major) embryos

doi:10.1016/j.theriogenology.2008.06.028

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	Effect of cryoprotectants on hatching rate of red seabream (Pagrus major) embryos
	Mao, Z. Z.1; Zhang, L. L.1,2; Xu, X. Z.; Liu, Q. H.1,2; Li, J.1 ; Ma, D. Y.1; Xu, S. H.1; Xue, Y. P.; Xue, Q. Z.3
	2008-10-15
发表期刊	THERIOGENOLOGY
ISSN	0093-691X
卷号	70 期号:7 页码:1086-1092
文章类型	Article
摘要	The objectives were to investigate the effect of cryoprotectants on the hatching rate of red seabream embryos. Heart-beat embryos were immersed in: five permeable cryoprotectants, dimethyl sulfoxide (DMSO), glycerol (Gly), methanol (MeOH), 1,2-propylene glycol (PG), and ethylene glycol (EG). in concentrations of 5-30% for 10, 30, or 60 min; and two non-permeable cryoprotectants: polyvinylpyrrolidone (PVP), and sucrose (in concentrations of 5-20% for 10 or 30 min). The embryos were then washed and incubated in filtered seawater until hatching occurred. The hatching rate of the embryos treated with permeable cryoprotectants decreased (P < 0.05) with increased concentration and duration of exposure. In addition, PG was the least toxic permeable cryoprotectant, followed by DMSO and EG, whereas Gly and MeOH were the most toxic. At a concentration of 15% and 30 min exposure, the hatching rate of the embryos immersed in PG was 93.3 +/- 7.0% (mean +/- S.D.), however. in DMSO. EG, Gly. and MeOH, it was 82.7 +/- 10.4, 22.0 +/- 5.7, 0.0 +/- 0.0, and 0.0 +/- 0.0%, respectively. Hatching rate of embryos treated with PVP decreased (P < 0.05) with the increase of concentration and exposure time, whereas for embryos treated with sucrose, there was no significant decrease in comparison with the control at the concentrations used. (C) 2008 Elsevier Inc. All rights reserved.; The objectives were to investigate the effect of cryoprotectants on the hatching rate of red seabream embryos. Heart-beat embryos were immersed in: five permeable cryoprotectants, dimethyl sulfoxide (DMSO), glycerol (Gly), methanol (MeOH), 1,2-propylene glycol (PG), and ethylene glycol (EG). in concentrations of 5-30% for 10, 30, or 60 min; and two non-permeable cryoprotectants: polyvinylpyrrolidone (PVP), and sucrose (in concentrations of 5-20% for 10 or 30 min). The embryos were then washed and incubated in filtered seawater until hatching occurred. The hatching rate of the embryos treated with permeable cryoprotectants decreased (P < 0.05) with increased concentration and duration of exposure. In addition, PG was the least toxic permeable cryoprotectant, followed by DMSO and EG, whereas Gly and MeOH were the most toxic. At a concentration of 15% and 30 min exposure, the hatching rate of the embryos immersed in PG was 93.3 +/- 7.0% (mean +/- S.D.), however. in DMSO. EG, Gly. and MeOH, it was 82.7 +/- 10.4, 22.0 +/- 5.7, 0.0 +/- 0.0, and 0.0 +/- 0.0%, respectively. Hatching rate of embryos treated with PVP decreased (P < 0.05) with the increase of concentration and exposure time, whereas for embryos treated with sucrose, there was no significant decrease in comparison with the control at the concentrations used. (C) 2008 Elsevier Inc. All rights reserved.
关键词	Red Seabream Embryo Cryoprotectant Toxicity Permeable And non-Permeable
学科领域	Reproductive Biology ; Veterinary Sciences
DOI	10.1016/j.theriogenology.2008.06.028
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000259750000009
引用统计	被引频次：15[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/6074
专题	海洋生物技术研发中心实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Ctr Biotechnol R&D, Qingdao 266071, Shandong, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100031, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res Sustainable Dev, Yantai 264003, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Mao, Z. Z.,Zhang, L. L.,Xu, X. Z.,et al. Effect of cryoprotectants on hatching rate of red seabream (Pagrus major) embryos[J]. THERIOGENOLOGY,2008,70(7):1086-1092.
APA	Mao, Z. Z..,Zhang, L. L..,Xu, X. Z..,Liu, Q. H..,Li, J..,...&Xue, Q. Z..(2008).Effect of cryoprotectants on hatching rate of red seabream (Pagrus major) embryos.THERIOGENOLOGY,70(7),1086-1092.
MLA	Mao, Z. Z.,et al."Effect of cryoprotectants on hatching rate of red seabream (Pagrus major) embryos".THERIOGENOLOGY 70.7(2008):1086-1092.

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