Species identification in salted products of red snappers by semi-nested PCR-RFLP based on the mitochondrial 12S rRNA gene sequence

doi:10.1016/j.foodcont.2005.01.011

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	Species identification in salted products of red snappers by semi-nested PCR-RFLP based on the mitochondrial 12S rRNA gene sequence
	Zhang, JB ; Huang, H ; Cai, ZP ; Huang, LM
	2006-07-01
发表期刊	FOOD CONTROL
ISSN	0956-7135
卷号	17 期号:7 页码:557-563
文章类型	Article
摘要	A molecular approach was developed to distinguish species of red snappers among commercial salted fish products. The specific fragments of the mitochondrial 12S rRNA gene, which were about 450bp, were obtained using the semi-nested polymerase chain reaction (semi-nested PCR). Subsequently, PCR arnplicons were sequenced, aiming to select restriction endonucleases that generated species-specific restriction fragment length polymorphism (RFLP) profiles. Discrimination of red snappers Lutjanus sanguineus, Lutjanus erythopterus from Lutjanus argentimaculatus, Lutjanus malabarius and other morphologically similar fishes such as Lethrinus leutjanus and Pinjalo pinjalo was feasible by one restriction digestion reaction with three endonucleases Hae III, Sca I and SnaB I, however, for discrimination of L. sanguineus and L. erythopterus, another restriction digestion reaction with single restriction endonuclease Mae II was needed. The semi-nested PCR-RFLP was demonstrated to be reliable in species identification of salted fish products in this study. (c) 2005 Elsevier Ltd. All rights reserved.; A molecular approach was developed to distinguish species of red snappers among commercial salted fish products. The specific fragments of the mitochondrial 12S rRNA gene, which were about 450bp, were obtained using the semi-nested polymerase chain reaction (semi-nested PCR). Subsequently, PCR arnplicons were sequenced, aiming to select restriction endonucleases that generated species-specific restriction fragment length polymorphism (RFLP) profiles. Discrimination of red snappers Lutjanus sanguineus, Lutjanus erythopterus from Lutjanus argentimaculatus, Lutjanus malabarius and other morphologically similar fishes such as Lethrinus leutjanus and Pinjalo pinjalo was feasible by one restriction digestion reaction with three endonucleases Hae III, Sca I and SnaB I, however, for discrimination of L. sanguineus and L. erythopterus, another restriction digestion reaction with single restriction endonuclease Mae II was needed. The semi-nested PCR-RFLP was demonstrated to be reliable in species identification of salted fish products in this study. (c) 2005 Elsevier Ltd. All rights reserved.
关键词	Identification Red Snappers Semi-nested Pcr 12s Rrna Gene
学科领域	Food Science & Technology
DOI	10.1016/j.foodcont.2005.01.011
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000235266600009
引用统计	被引频次：20[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/5984
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, S China Sea Inst Oceanog, LED, Guangzhou 510301, Peoples R China 2.Chinese Acad Sci, Inst Oceanog, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Zhang, JB,Huang, H,Cai, ZP,et al. Species identification in salted products of red snappers by semi-nested PCR-RFLP based on the mitochondrial 12S rRNA gene sequence[J]. FOOD CONTROL,2006,17(7):557-563.
APA	Zhang, JB,Huang, H,Cai, ZP,&Huang, LM.(2006).Species identification in salted products of red snappers by semi-nested PCR-RFLP based on the mitochondrial 12S rRNA gene sequence.FOOD CONTROL,17(7),557-563.
MLA	Zhang, JB,et al."Species identification in salted products of red snappers by semi-nested PCR-RFLP based on the mitochondrial 12S rRNA gene sequence".FOOD CONTROL 17.7(2006):557-563.

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