Differentiation of the rainbow trout (Oncorhynchus mykiss) from Atlantic salmon (Salmon salar) by the AFLP-derived SCAR

doi:10.1007/s00217-005-0221-9

IOCAS-IR > 实验海洋生物学重点实验室

	Differentiation of the rainbow trout (Oncorhynchus mykiss) from Atlantic salmon (Salmon salar) by the AFLP-derived SCAR
	Zhang, JB ; Cai, ZP
	2006-07-01
发表期刊	EUROPEAN FOOD RESEARCH AND TECHNOLOGY
ISSN	1438-2377
卷号	223 期号:3 页码:413-417
文章类型	Article
摘要	A species-specific SCAR marker for rainbow trout, which was used to detect adulteration and fraudulent labeling in Atlantic salmon products, has been developed based on the AFLP analysis and evaluated in this study. The SCAR marker could be amplified and visualized in 1% agarose gel in all tested rainbow trout samples and absent in all salmon samples. Using DNA admixtures, the detection of 1% (0.5 ng), 10% (5 ng) rainbow trout DNA in Atlantic salmon DNA for fresh and processed samples, respectively was readily achieved. The molecular approach was sensitive and demonstrated to be a rapid and reliable method for identifying frauds in salmon products and could be extended for applications of species identification in food industry.; A species-specific SCAR marker for rainbow trout, which was used to detect adulteration and fraudulent labeling in Atlantic salmon products, has been developed based on the AFLP analysis and evaluated in this study. The SCAR marker could be amplified and visualized in 1% agarose gel in all tested rainbow trout samples and absent in all salmon samples. Using DNA admixtures, the detection of 1% (0.5 ng), 10% (5 ng) rainbow trout DNA in Atlantic salmon DNA for fresh and processed samples, respectively was readily achieved. The molecular approach was sensitive and demonstrated to be a rapid and reliable method for identifying frauds in salmon products and could be extended for applications of species identification in food industry.
关键词	Identification Atlantic Salmon Rainbow Trout Aflp Scar
学科领域	Food Science & Technology
DOI	10.1007/s00217-005-0221-9
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000238292800016
引用统计	被引频次：15[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/5978
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, AMBL&LED, S China Sea Inst Oceanog, Guangzhou 510301, Peoples R China 2.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanog, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Zhang, JB,Cai, ZP. Differentiation of the rainbow trout (Oncorhynchus mykiss) from Atlantic salmon (Salmon salar) by the AFLP-derived SCAR[J]. EUROPEAN FOOD RESEARCH AND TECHNOLOGY,2006,223(3):413-417.
APA	Zhang, JB,&Cai, ZP.(2006).Differentiation of the rainbow trout (Oncorhynchus mykiss) from Atlantic salmon (Salmon salar) by the AFLP-derived SCAR.EUROPEAN FOOD RESEARCH AND TECHNOLOGY,223(3),413-417.
MLA	Zhang, JB,et al."Differentiation of the rainbow trout (Oncorhynchus mykiss) from Atlantic salmon (Salmon salar) by the AFLP-derived SCAR".EUROPEAN FOOD RESEARCH AND TECHNOLOGY 223.3(2006):413-417.

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