Genetic analysis of the gametophytes of Undaria pinnatifida (Phaeophyceae) with ISSR method

doi:10.1016/j.aquaculture.2006.04.029

IOCAS-IR > 实验海洋生物学重点实验室

	Genetic analysis of the gametophytes of Undaria pinnatifida (Phaeophyceae) with ISSR method
	Wang, Xuliang; Wang, Di; Li, Dapeng; Duan, Delin
	2006-08-31
发表期刊	AQUACULTURE
ISSN	0044-8486
卷号	258 期号:1-4 页码:250-256
文章类型	Article
摘要	Inter-simple sequence repeat (ISSR) analysis was used to assess eleven pairs of Undaria pinnatifida (Harv.) Suringar male and female gametophytes. After screening fifty primers, 18 ISSR primers were selected for final analysis. A total of 104 loci were obtained, of which 77 were polymorphic, among the gametophytes studied. Genetic relationships were analyzed with simple matching (S), Jaccard's (J) and Dice's (D) distance coefficients. Little genetic variations were found among the selected Undaria gametophytes, for instance, the genetic distances ranging from 0.010 to 0.125 with Dice coefficients. UPGMA dendrograms showed that 11 pairs of Undaria gametophytes were distributed into five groups. Most Undaria strains cultivated in China exhibited closely genetic relationships with the strains from Japan. However, gametophytes from Qingdao appeared as distinct clades from other Undaria strains with all three distance coefficients used. Mantel test showed that the three distance measurements generated congruent clustering patterns on the same data. Our results demonstrated the feasibility of applying ISSR markers for genetic analysis of Undaria gametophytes. (c) 2006 Elsevier B.V. All rights reserved.; Inter-simple sequence repeat (ISSR) analysis was used to assess eleven pairs of Undaria pinnatifida (Harv.) Suringar male and female gametophytes. After screening fifty primers, 18 ISSR primers were selected for final analysis. A total of 104 loci were obtained, of which 77 were polymorphic, among the gametophytes studied. Genetic relationships were analyzed with simple matching (S), Jaccard's (J) and Dice's (D) distance coefficients. Little genetic variations were found among the selected Undaria gametophytes, for instance, the genetic distances ranging from 0.010 to 0.125 with Dice coefficients. UPGMA dendrograms showed that 11 pairs of Undaria gametophytes were distributed into five groups. Most Undaria strains cultivated in China exhibited closely genetic relationships with the strains from Japan. However, gametophytes from Qingdao appeared as distinct clades from other Undaria strains with all three distance coefficients used. Mantel test showed that the three distance measurements generated congruent clustering patterns on the same data. Our results demonstrated the feasibility of applying ISSR markers for genetic analysis of Undaria gametophytes. (c) 2006 Elsevier B.V. All rights reserved.
关键词	Undaria Gametophytes Inter-simple Sequence Repeat Genetic Analysis Mantel Test Dendrogram
学科领域	Fisheries ; Marine & Freshwater Biology
DOI	10.1016/j.aquaculture.2006.04.029
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000240252900026
引用统计	被引频次：17[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/5956
专题	实验海洋生物学重点实验室
作者单位	Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Wang, Xuliang,Wang, Di,Li, Dapeng,et al. Genetic analysis of the gametophytes of Undaria pinnatifida (Phaeophyceae) with ISSR method[J]. AQUACULTURE,2006,258(1-4):250-256.
APA	Wang, Xuliang,Wang, Di,Li, Dapeng,&Duan, Delin.(2006).Genetic analysis of the gametophytes of Undaria pinnatifida (Phaeophyceae) with ISSR method.AQUACULTURE,258(1-4),250-256.
MLA	Wang, Xuliang,et al."Genetic analysis of the gametophytes of Undaria pinnatifida (Phaeophyceae) with ISSR method".AQUACULTURE 258.1-4(2006):250-256.

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