Physiological and immune responses of zhikong scallop Chlamys farreri to the acute viral necrobiotic virus infection

doi:10.1016/j.fsi.2010.02.019

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	Physiological and immune responses of zhikong scallop Chlamys farreri to the acute viral necrobiotic virus infection
	Tang, Baojun 1,2; Liu, Baozhong1 ; Wang, Xiaomei 1; Yue, Xin 1; Xiang, Jianhai1
	2010-07-01
发表期刊	FISH & SHELLFISH IMMUNOLOGY
ISSN	1050-4648
卷号	29 期号:1 页码:42-48
文章类型	Article
摘要	The Zhikong Scallop, Chlamys farreri, is one of the most Important bivalve mollusks cultured in northern China However, mass mortality of the cultured C farreri has posed a serious threat to the maricultural Industry in recent years. Acute Viral Necrobiotic Virus (AVNV) is believed as an important etiological agent causing the scallop mass mortalities To understand the mechanism behind the AVNV associated scallop disease and mortality, we assessed the physiological and immune responses of C farreri to the virus infection using oxygen consumption rate, ammonium-nitrogen excretion rate, hemocyte copper, zinc superoxide dismutase gene expression, and plasma superoxide dismutase activity and alkaline phosphatase activity as indicators Scallops challenged by AVNV at 25 C developed typical disease signs 2 days after virus injection Before the disease manifested, scallop oxygen consumption and NH(4)(+)-N excretion rates rose and then fell back. Real-time PCR revealed that the hemocyte cytosol Cu, Zn SOD gene expression was upregulated followed by recovery The plasma SOD activity, however, augmented consistently following virus injection Moreover, plasma AKP activity first lowered and then elevated gradually to the highest level at 24 h post virus injection Scallops challenged by AVNV at 17 degrees C neither developed notable disease nor showed obvious responses that could be associated with the virus infection. While the results suggested a correlation between the elevated seawater temperature and the AVNV infection associated C farreri mortalities, they also indicated that the viral infection provoked multiple physiological and immune responses in the host scallops (C) 2010 Elsevier Ltd All rights reserved; The Zhikong Scallop, Chlamys farreri, is one of the most Important bivalve mollusks cultured in northern China However, mass mortality of the cultured C farreri has posed a serious threat to the maricultural Industry in recent years. Acute Viral Necrobiotic Virus (AVNV) is believed as an important etiological agent causing the scallop mass mortalities To understand the mechanism behind the AVNV associated scallop disease and mortality, we assessed the physiological and immune responses of C farreri to the virus infection using oxygen consumption rate, ammonium-nitrogen excretion rate, hemocyte copper, zinc superoxide dismutase gene expression, and plasma superoxide dismutase activity and alkaline phosphatase activity as indicators Scallops challenged by AVNV at 25 C developed typical disease signs 2 days after virus injection Before the disease manifested, scallop oxygen consumption and NH4+-N excretion rates rose and then fell back. Real-time PCR revealed that the hemocyte cytosol Cu, Zn SOD gene expression was upregulated followed by recovery The plasma SOD activity, however, augmented consistently following virus injection Moreover, plasma AKP activity first lowered and then elevated gradually to the highest level at 24 h post virus injection Scallops challenged by AVNV at 17 degrees C neither developed notable disease nor showed obvious responses that could be associated with the virus infection. While the results suggested a correlation between the elevated seawater temperature and the AVNV infection associated C farreri mortalities, they also indicated that the viral infection provoked multiple physiological and immune responses in the host scallops (C) 2010 Elsevier Ltd All rights reserved
关键词	Chlamys Farreri Acute Viral Necrobiotic Virus Physiological Immune Response
学科领域	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
DOI	10.1016/j.fsi.2010.02.019
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000278649000004
引用统计	被引频次：33[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/5932
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Fishery Sci, E China Sea Fisheries Res Inst, Shanghai 200090, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Tang, Baojun,Liu, Baozhong,Wang, Xiaomei,et al. Physiological and immune responses of zhikong scallop Chlamys farreri to the acute viral necrobiotic virus infection[J]. FISH & SHELLFISH IMMUNOLOGY,2010,29(1):42-48.
APA	Tang, Baojun,Liu, Baozhong,Wang, Xiaomei,Yue, Xin,&Xiang, Jianhai.(2010).Physiological and immune responses of zhikong scallop Chlamys farreri to the acute viral necrobiotic virus infection.FISH & SHELLFISH IMMUNOLOGY,29(1),42-48.
MLA	Tang, Baojun,et al."Physiological and immune responses of zhikong scallop Chlamys farreri to the acute viral necrobiotic virus infection".FISH & SHELLFISH IMMUNOLOGY 29.1(2010):42-48.

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