Purification and characterization of two types of chitosanase from a Microbacterium sp.

doi:10.1007/s10529-006-9101-z

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	Purification and characterization of two types of chitosanase from a Microbacterium sp.
	Sun, Yuying ; Liu, Wanshun ; Han, Baoqin ; Zhang, Jiquan ; Liu, Bing
	2006-09-01
发表期刊	BIOTECHNOLOGY LETTERS
ISSN	0141-5492
卷号	28 期号:17 页码:1393-1399
文章类型	Article
摘要	Two extracellular chitosanases (ChiX and ChiN) were extracted from Microbacterium sp. OU01 with Mr values of 81 kDa (ChiX) and 30 kDa (ChiN). ChiN was optimally active at pH 6.2 and 50 degrees C and ChiX at pH 6.6 and 60 degrees C (assayed over 15 min). Both the activities increased with the degree of deacetylation (DDA) of chitosan. ChiN hydrolyzed oligomers of glucosamine (GlcN) larger than chitopentaose, and chitosan with 62-100% DDA; but ChiX acted on chitosan and released GlcN. Hydrolysis of chitosan with 99% DDA by ChiN released chitobiose, chitotriose and chitotetraose as the major products.; Two extracellular chitosanases (ChiX and ChiN) were extracted from Microbacterium sp. OU01 with Mr values of 81 kDa (ChiX) and 30 kDa (ChiN). ChiN was optimally active at pH 6.2 and 50 degrees C and ChiX at pH 6.6 and 60 degrees C (assayed over 15 min). Both the activities increased with the degree of deacetylation (DDA) of chitosan. ChiN hydrolyzed oligomers of glucosamine (GlcN) larger than chitopentaose, and chitosan with 62-100% DDA; but ChiX acted on chitosan and released GlcN. Hydrolysis of chitosan with 99% DDA by ChiN released chitobiose, chitotriose and chitotetraose as the major products.
关键词	Chitooligosaccharides Chitosan Chitosanase Endo-type Exo-type Microbacterium Sp.
学科领域	Biotechnology & Applied Microbiology
DOI	10.1007/s10529-006-9101-z
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000239813900011
引用统计	被引频次：33[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/5926
专题	实验海洋生物学重点实验室
作者单位	1.Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Sun, Yuying,Liu, Wanshun,Han, Baoqin,et al. Purification and characterization of two types of chitosanase from a Microbacterium sp.[J]. BIOTECHNOLOGY LETTERS,2006,28(17):1393-1399.
APA	Sun, Yuying,Liu, Wanshun,Han, Baoqin,Zhang, Jiquan,&Liu, Bing.(2006).Purification and characterization of two types of chitosanase from a Microbacterium sp..BIOTECHNOLOGY LETTERS,28(17),1393-1399.
MLA	Sun, Yuying,et al."Purification and characterization of two types of chitosanase from a Microbacterium sp.".BIOTECHNOLOGY LETTERS 28.17(2006):1393-1399.

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