Settling abalone veliger larvae in a free-swimming microalgal culture

doi:10.1016/j.aquaculture.2006.03.028

IOCAS-IR > 实验海洋生物学重点实验室

	Settling abalone veliger larvae in a free-swimming microalgal culture
	Pang, S. J.; Zhang, Z. H.; Bao, Y.; Gao, S. Q.
	2006-08-31
发表期刊	AQUACULTURE
ISSN	0044-8486
卷号	258 期号:1-4 页码:327-336
文章类型	Article
摘要	In the current abalone hatchery in China, insufficient diatoms on vertically placed corrugated pvc plates at later stage often could not support the growth of postlarvae up to the stage that they can feed on live macroalgae. As a result, stripping the spats (35 mm) off by anaesthetization and switching the diet from live diatoms to artificial powdered diet in combination has to be performed in most of the abalone farms. This manipulation normally leads to more than 50% mortality. Here we report the direct use of the unicellular green alga Platymonas helgolandica Kylin var. tsingtaoensis as a potential alga to be used to settle the veliger larvae of the Pacific abalone Haliotis discus hannai and to feed the postlarvae. Settlement rate of 2-day-old veliger larvae in mono culture of P helgolandica could be as high as 92% ( +/- 4.2%) on day 10 in small scale trials, higher than that in the selected benthic diatom strain (53.6% +/- 12.7%) when settled in the water in which bacteria propagation was controlled by treatment of 2 ppm of benzylpenicillinum calcium and streptomycin sulfate. Postlarvae fed solely on P. helgolandica or the selected benthic diatom Navicula-2005-A grew at rates of 40.1 ( +/- 1.9) and 45.8 (+/- 13.4) mu m day(-1), respectively, when raised at 22 degrees C until day 50 postfertilization. P. helgolandica was shown to have distinct diurnal settling rhythm characterized with a peak of settled cells in the middle of the night for cell division and a peak of free-swimming cells in the middle of the day. High density of attached P. helgolandica cells on the inner surface of the culture facility in the night fits the nocturnal feeding behavior of the abalone spats. Judged by the promising larvae settling rate, growth and survival rates of the postlarvae fed with this alga, the free-swimming micro-green alga P. helgolandica constitutes a potential species for settling the veliger larvae and for supporting the growth of postlarvae as well. (c) 2006 Published by Elsevier B.V.; In the current abalone hatchery in China, insufficient diatoms on vertically placed corrugated pvc plates at later stage often could not support the growth of postlarvae up to the stage that they can feed on live macroalgae. As a result, stripping the spats (35 mm) off by anaesthetization and switching the diet from live diatoms to artificial powdered diet in combination has to be performed in most of the abalone farms. This manipulation normally leads to more than 50% mortality. Here we report the direct use of the unicellular green alga Platymonas helgolandica Kylin var. tsingtaoensis as a potential alga to be used to settle the veliger larvae of the Pacific abalone Haliotis discus hannai and to feed the postlarvae. Settlement rate of 2-day-old veliger larvae in mono culture of P helgolandica could be as high as 92% ( +/- 4.2%) on day 10 in small scale trials, higher than that in the selected benthic diatom strain (53.6% +/- 12.7%) when settled in the water in which bacteria propagation was controlled by treatment of 2 ppm of benzylpenicillinum calcium and streptomycin sulfate. Postlarvae fed solely on P. helgolandica or the selected benthic diatom Navicula-2005-A grew at rates of 40.1 ( +/- 1.9) and 45.8 (+/- 13.4) mu m day(-1), respectively, when raised at 22 degrees C until day 50 postfertilization. P. helgolandica was shown to have distinct diurnal settling rhythm characterized with a peak of settled cells in the middle of the night for cell division and a peak of free-swimming cells in the middle of the day. High density of attached P. helgolandica cells on the inner surface of the culture facility in the night fits the nocturnal feeding behavior of the abalone spats. Judged by the promising larvae settling rate, growth and survival rates of the postlarvae fed with this alga, the free-swimming micro-green alga P. helgolandica constitutes a potential species for settling the veliger larvae and for supporting the growth of postlarvae as well. (c) 2006 Published by Elsevier B.V.
关键词	Haliotis Discus Hannai Platymonas Helgolandica Kylin Var. Tsingtaoensis Veliger Larvae Abalone Hatchery
学科领域	Fisheries ; Marine & Freshwater Biology
DOI	10.1016/j.aquaculture.2006.03.028
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000240252900036
引用统计	被引频次：8[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/5892
专题	实验海洋生物学重点实验室
作者单位	Chinese Acad Sci, Inst Oceanol, Marine Biol Culture Collect Ctr, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Pang, S. J.,Zhang, Z. H.,Bao, Y.,et al. Settling abalone veliger larvae in a free-swimming microalgal culture[J]. AQUACULTURE,2006,258(1-4):327-336.
APA	Pang, S. J.,Zhang, Z. H.,Bao, Y.,&Gao, S. Q..(2006).Settling abalone veliger larvae in a free-swimming microalgal culture.AQUACULTURE,258(1-4),327-336.
MLA	Pang, S. J.,et al."Settling abalone veliger larvae in a free-swimming microalgal culture".AQUACULTURE 258.1-4(2006):327-336.

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