There are two 5 '-flanking regions of bkt encoding beta-carotene ketolase in Haematococcus pluvialis

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	There are two 5 '-flanking regions of bkt encoding beta-carotene ketolase in Haematococcus pluvialis
	Meng, CX ; Liang, CW ; Su, ZL ; Qin, S ; Tseng, CK
	2006-03-01
发表期刊	PHYCOLOGIA
ISSN	0031-8884
卷号	45 期号:2 页码:218-224
文章类型	Article
摘要	The unicellular green alga Haematococcus pluvialis accumulates a commercially valuable astaxanthin, with levels reaching up to 4% dry weight under environmental stress. In recent years, much effort has been devoted to understanding the molecular mechanisms regulating astaxanthin biosynthetic pathways. Beta-carotene ketolase (bkt), with control being exhibited at the transcription level, plays an important role in astaxanthin biosynthesis by H. pluvialis. Here we demonstrate the presence of two separate 5'-flanking regions [1.5 kilobase (kb) and 2 kb] of bkt (bkt1 and bkt2) that possess regulatory elements similar to those of known stress-responsive genes in plants. Results of 5'-deletion constructs and transient beta-galactosidase expression assays demonstrate that there may be positive regulatory elements governing expression in the shorter promoter at -1060/-900 from the 1.5 kb 5' region, and in the longer promoter at -1838/-1219 and at -1046/ -734 from the 2 kb 5' region relative to each homologous ATG start codon. Furthermore, our present studies reveal that the first intron (+371/+497) downstream from the 1.5 kb 5' untranslated region of bkt1 may function as a negative regulatory element to regulate its own promoter.; The unicellular green alga Haematococcus pluvialis accumulates a commercially valuable astaxanthin, with levels reaching up to 4% dry weight under environmental stress. In recent years, much effort has been devoted to understanding the molecular mechanisms regulating astaxanthin biosynthetic pathways. Beta-carotene ketolase (bkt), with control being exhibited at the transcription level, plays an important role in astaxanthin biosynthesis by H. pluvialis. Here we demonstrate the presence of two separate 5'-flanking regions [1.5 kilobase (kb) and 2 kb] of bkt (bkt1 and bkt2) that possess regulatory elements similar to those of known stress-responsive genes in plants. Results of 5'-deletion constructs and transient beta-galactosidase expression assays demonstrate that there may be positive regulatory elements governing expression in the shorter promoter at -1060/-900 from the 1.5 kb 5' region, and in the longer promoter at -1838/-1219 and at -1046/ -734 from the 2 kb 5' region relative to each homologous ATG start codon. Furthermore, our present studies reveal that the first intron (+371/+497) downstream from the 1.5 kb 5' untranslated region of bkt1 may function as a negative regulatory element to regulate its own promoter.
关键词	Haematococcus Pluvialis Astaxanthin Beta-carotene Ketolase 5 '-flanking Region Regulatory Elements Deletion Analysis
学科领域	Plant Sciences ; Marine & Freshwater Biology
收录类别	SCI
语种	英语
WOS记录号	WOS:000235940800012
引用统计	被引频次：12[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/5876
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Zhejiang Univ, Coll Life Sci, Dept Biotechnol, Hangzhou 310027, Peoples R China
推荐引用方式 GB/T 7714	Meng, CX,Liang, CW,Su, ZL,et al. There are two 5 '-flanking regions of bkt encoding beta-carotene ketolase in Haematococcus pluvialis[J]. PHYCOLOGIA,2006,45(2):218-224.
APA	Meng, CX,Liang, CW,Su, ZL,Qin, S,&Tseng, CK.(2006).There are two 5 '-flanking regions of bkt encoding beta-carotene ketolase in Haematococcus pluvialis.PHYCOLOGIA,45(2),218-224.
MLA	Meng, CX,et al."There are two 5 '-flanking regions of bkt encoding beta-carotene ketolase in Haematococcus pluvialis".PHYCOLOGIA 45.2(2006):218-224.

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