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| Isolation and characterization of the oxygen-evolving photosystem II complex from the economical red alga Bangia fusco-purpurea | |
| Li, Y. X.1; Gao, Z. Q.2; Li, D. M.3; Wang, G. C.4; Wang, F.1 | |
| 2010-09-01 | |
| 发表期刊 | RUSSIAN JOURNAL OF PLANT PHYSIOLOGY
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| ISSN | 1021-4437 |
| 卷号 | 57期号:5页码:609-614 |
| 文章类型 | Article |
| 摘要 | The highly pure and active photosystem II (PSII) complex was isolated from Bangia fusco-purpurea (Dillw) Lyngb., an important economic red alga in China, through two steps of sucrose density gradient ultracentrifugation and characterized by the room absorption and fluorescence emission spectra, DCIP (2,6-dichloroindophenol) reduction, and oxygen evolution rates. The PSII complex from B. fusco-purpurea had the characteristic absorption peaks of chlorophyll (Chl) a (436 and 676 nm) and typical fluorescence emission peak at 685 nm (Ex = 436 nm). Moreover, the acquired PSII complex displayed high oxygen evolution (139 mu mol O(2)/(mg Chl h) in the presence of 2.5 mM 2,6-dimethybenzoqinone as an artificial acceptor and was active in photoreduction of DCIP (2,6-dichloroindophenol) by DPC (1,5-diphenylcarbazide) at 163 U/(mg Chl a h). SDS-PAGE also suggested that the purified PSII complex contained four intrinsic proteins (D1, D2, CP43, and CP47) and four extrinsic proteins (33-kD protein, 20-kD protein, cyt c-550, and 14-kD protein).; The highly pure and active photosystem II (PSII) complex was isolated from Bangia fusco-purpurea (Dillw) Lyngb., an important economic red alga in China, through two steps of sucrose density gradient ultracentrifugation and characterized by the room absorption and fluorescence emission spectra, DCIP (2,6-dichloroindophenol) reduction, and oxygen evolution rates. The PSII complex from B. fusco-purpurea had the characteristic absorption peaks of chlorophyll (Chl) a (436 and 676 nm) and typical fluorescence emission peak at 685 nm (Ex = 436 nm). Moreover, the acquired PSII complex displayed high oxygen evolution (139 mu mol O-2/(mg Chl h) in the presence of 2.5 mM 2,6-dimethybenzoqinone as an artificial acceptor and was active in photoreduction of DCIP (2,6-dichloroindophenol) by DPC (1,5-diphenylcarbazide) at 163 U/(mg Chl a h). SDS-PAGE also suggested that the purified PSII complex contained four intrinsic proteins (D1, D2, CP43, and CP47) and four extrinsic proteins (33-kD protein, 20-kD protein, cyt c-550, and 14-kD protein). |
| 关键词 | Bangia Fusco-purpurea Photosystem Ii Fluorescence Emission Spectra Oxygen Evolution |
| 学科领域 | Plant Sciences |
| DOI | 10.1134/S102144371005002X |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000281543600002 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/5852 |
| 专题 | 实验海洋生物学重点实验室 海洋环流与波动重点实验室 |
| 作者单位 | 1.Nanyang Normal Univ, Sch Life Sci & Technol, Nanyang 473061, Peoples R China 2.Shandong Univ Technol, Sch Life Sci, Zibo 255049, Peoples R China 3.Liaocheng Univ, Coll Agr, Liaocheng 252059, Peoples R China 4.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Y. X.,Gao, Z. Q.,Li, D. M.,et al. Isolation and characterization of the oxygen-evolving photosystem II complex from the economical red alga Bangia fusco-purpurea[J]. RUSSIAN JOURNAL OF PLANT PHYSIOLOGY,2010,57(5):609-614. |
| APA | Li, Y. X.,Gao, Z. Q.,Li, D. M.,Wang, G. C.,&Wang, F..(2010).Isolation and characterization of the oxygen-evolving photosystem II complex from the economical red alga Bangia fusco-purpurea.RUSSIAN JOURNAL OF PLANT PHYSIOLOGY,57(5),609-614. |
| MLA | Li, Y. X.,et al."Isolation and characterization of the oxygen-evolving photosystem II complex from the economical red alga Bangia fusco-purpurea".RUSSIAN JOURNAL OF PLANT PHYSIOLOGY 57.5(2010):609-614. |
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