Identification and molecular characterization of a peritrophin-like protein from fleshy prawn (Fenneropenaeus chinensis)

doi:10.1016/j.molimm.2005.09.018

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	Identification and molecular characterization of a peritrophin-like protein from fleshy prawn (Fenneropenaeus chinensis)
	Du, XJ ; Wang, JX; Liu, N ; Zhao, XF ; Li, FH ; Xiang, JH
	2006-04-01
发表期刊	MOLECULAR IMMUNOLOGY
ISSN	0161-5890
卷号	43 期号:10 页码:1633-1644
文章类型	Article
摘要	Peritrophin, one of the components of the peritrophic matrix, was first isolated from the intestine of insects. It is thought to protect insects from invasion of microorganisms and to stimulate digestion of food. Peritrophin-like proteins have also been found in crustaceans, as a component of the egg layer. In this study, one fragment of the peritrophin-like gene was obtained from fleshy prawn (Chinese shrimp) (Fenneropenaeus chinensis) by panning the T7 phage display library constructed with the shrimp hemocyte cDNA. The total sequence of the peritrophin cDNA was cloned by modified SMART cDNA and LD-PCR methods. The full cDNA is 1048 bp and the deduced protein is composed of 274 amino acids, including 21 amino acid signal peptide, and four peritrophin A domains and the latter three forming three chitin-binding domains. Similarity analysis results showed that the peritrophin-like protein from F chinensis has significant similarities with peritrophin-like and cortical rod proteins from other shrimp. It was inducing expression in hemocytes, heart, stomach, gut, and gills of the infected shrimp, and constitutive expression in the ovaries. No expression signal was detected in the hepatopancreas of either infected or noninfected shrimp. The recombinant peritrophin-like protein has the activity of binding Gram-negative bacteria and strong binding activity to chitin. Therefore, the bacteria and chitin binding activities of the peritrophin-like protein suggest that it may plays a role in immune defense and other physiological resposes. (c) 2005 Elsevier Ltd. All rights reserved.; Peritrophin, one of the components of the peritrophic matrix, was first isolated from the intestine of insects. It is thought to protect insects from invasion of microorganisms and to stimulate digestion of food. Peritrophin-like proteins have also been found in crustaceans, as a component of the egg layer. In this study, one fragment of the peritrophin-like gene was obtained from fleshy prawn (Chinese shrimp) (Fenneropenaeus chinensis) by panning the T7 phage display library constructed with the shrimp hemocyte cDNA. The total sequence of the peritrophin cDNA was cloned by modified SMART cDNA and LD-PCR methods. The full cDNA is 1048 bp and the deduced protein is composed of 274 amino acids, including 21 amino acid signal peptide, and four peritrophin A domains and the latter three forming three chitin-binding domains. Similarity analysis results showed that the peritrophin-like protein from F chinensis has significant similarities with peritrophin-like and cortical rod proteins from other shrimp. It was inducing expression in hemocytes, heart, stomach, gut, and gills of the infected shrimp, and constitutive expression in the ovaries. No expression signal was detected in the hepatopancreas of either infected or noninfected shrimp. The recombinant peritrophin-like protein has the activity of binding Gram-negative bacteria and strong binding activity to chitin. Therefore, the bacteria and chitin binding activities of the peritrophin-like protein suggest that it may plays a role in immune defense and other physiological resposes. (c) 2005 Elsevier Ltd. All rights reserved.
关键词	Peritrophin-like Gene Expression Pattern Fleshy Prawn Fenneropenaeus Chinensis Chitin Binding Activity Immune Defense
学科领域	Biochemistry & Molecular Biology ; Immunology
DOI	10.1016/j.molimm.2005.09.018
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000236450900015
引用统计	被引频次：80[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/5820
专题	实验海洋生物学重点实验室
作者单位	1.Shandong Univ, Sch Life Sci, Jinan 250100, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Du, XJ,Wang, JX,Liu, N,et al. Identification and molecular characterization of a peritrophin-like protein from fleshy prawn (Fenneropenaeus chinensis)[J]. MOLECULAR IMMUNOLOGY,2006,43(10):1633-1644.
APA	Du, XJ,Wang, JX,Liu, N,Zhao, XF,Li, FH,&Xiang, JH.(2006).Identification and molecular characterization of a peritrophin-like protein from fleshy prawn (Fenneropenaeus chinensis).MOLECULAR IMMUNOLOGY,43(10),1633-1644.
MLA	Du, XJ,et al."Identification and molecular characterization of a peritrophin-like protein from fleshy prawn (Fenneropenaeus chinensis)".MOLECULAR IMMUNOLOGY 43.10(2006):1633-1644.

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