Evaluation of antioxidant property of extract and fractions obtained from a red alga, Polysiphonia urceolata

doi:10.1016/j.foodchem.2004.12.015

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	Evaluation of antioxidant property of extract and fractions obtained from a red alga, Polysiphonia urceolata
	Duan, XJ; Zhang, WW; Li, XM; Wang, BG
	2006-03-01
发表期刊	FOOD CHEMISTRY
ISSN	0308-8146
卷号	95 期号:1 页码:37-43
文章类型	Article
摘要	Antioxidant activity (AA), total phenolic content, and reducing power of the crude extract, fractions, and subfractions derived from a red alga, Polysiphonia urceolata, were evaluated and determined. The antioxidative activity was measured using the alpha,alpha-diphenyl-beta-pierylhydrazyl (DPPH) radical scavenging assay and the P-carotene-linoleate assay systems, and compared with that of butylated hydroxytoluene (BHT), gallic acid (GA), and ascorbic acid (AscA). The results showed that the crude extract and the ethyl acetate-soluble fraction exhibited higher AA than BHT in the DPPH assay model, at all of four concentration levels tested (from 0.4 to 50 mu g/ml), while, in the beta-carotene-linoleate assay system, the crude extract and the ethyl acetate-soluble fraction exhibited similar or, in most cases, higher AA than GA and AscA at the same concentrations (from 10 to 200 mu g/ml). The ethyl acetate-soluble fraction was further fractionated into seven subfractions F1-F7 by silica gel vacuum liquid chromatography. F1 was found to be the most effective subfraction in both assay systems. The total phenolic content and reducing power were determined using the Folin-Ciocalteu and the potassium ferricyanide reduction methods, respectively. Statistical analysis indicated a significant association between the antioxidant potency and total phenolic content as well as between the antioxidant potency and reducing power. (c) 2005 Elsevier Ltd. All rights reserved.; Antioxidant activity (AA), total phenolic content, and reducing power of the crude extract, fractions, and subfractions derived from a red alga, Polysiphonia urceolata, were evaluated and determined. The antioxidative activity was measured using the alpha,alpha-diphenyl-beta-pierylhydrazyl (DPPH) radical scavenging assay and the P-carotene-linoleate assay systems, and compared with that of butylated hydroxytoluene (BHT), gallic acid (GA), and ascorbic acid (AscA). The results showed that the crude extract and the ethyl acetate-soluble fraction exhibited higher AA than BHT in the DPPH assay model, at all of four concentration levels tested (from 0.4 to 50 mu g/ml), while, in the beta-carotene-linoleate assay system, the crude extract and the ethyl acetate-soluble fraction exhibited similar or, in most cases, higher AA than GA and AscA at the same concentrations (from 10 to 200 mu g/ml). The ethyl acetate-soluble fraction was further fractionated into seven subfractions F1-F7 by silica gel vacuum liquid chromatography. F1 was found to be the most effective subfraction in both assay systems. The total phenolic content and reducing power were determined using the Folin-Ciocalteu and the potassium ferricyanide reduction methods, respectively. Statistical analysis indicated a significant association between the antioxidant potency and total phenolic content as well as between the antioxidant potency and reducing power. (c) 2005 Elsevier Ltd. All rights reserved.
关键词	Polysiphonia Urceolata Antioxidant Dpph Beta-carotene-linoleate Total Phenolic Content Reducing Power
学科领域	Chemistry, Applied ; Food Science & Technology ; Nutrition & Dietetics
DOI	10.1016/j.foodchem.2004.12.015
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000232762000005
引用统计	被引频次：435[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/5818
专题	实验海洋生物学重点实验室
作者单位	Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Duan, XJ,Zhang, WW,Li, XM,et al. Evaluation of antioxidant property of extract and fractions obtained from a red alga, Polysiphonia urceolata[J]. FOOD CHEMISTRY,2006,95(1):37-43.
APA	Duan, XJ,Zhang, WW,Li, XM,&Wang, BG.(2006).Evaluation of antioxidant property of extract and fractions obtained from a red alga, Polysiphonia urceolata.FOOD CHEMISTRY,95(1),37-43.
MLA	Duan, XJ,et al."Evaluation of antioxidant property of extract and fractions obtained from a red alga, Polysiphonia urceolata".FOOD CHEMISTRY 95.1(2006):37-43.

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