A novel polypeptide from shark cartilage with potent anti-angiogenic activity

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	A novel polypeptide from shark cartilage with potent anti-angiogenic activity
	Zheng, Lanhong ; Ling, Peixue ; Wang, Zheng ; Niu, Rongli ; Hu, Chaoxin ; Zhang, Tianmin ; Lin, Xiukun
	2007-05-01
发表期刊	CANCER BIOLOGY & THERAPY
ISSN	1538-4047
卷号	6 期号:5 页码:775-780
文章类型	Article
摘要	Using guanidine-HCl extraction, acetone precipitation, ultra-filtration and chromatography, a novel polypeptide with potent anti-angiogenic activity was purified from cartilage of the shark, Prionace glauca. N-terminal amino acid sequence analysis and SDS-PAGE revealed that the substance is a novel polypeptide with MW 15500 (PG155). The anti-angiogenic effects of PG155 were evaluated using zebrafish embryos model in vivo. Treatment of the embryos with 20 mu g/ml PG155 resulted in a significant reduction in the growth of subintestinal vessels (SIVs). A higher dose resulted in almost complete inhibition of SIV growth, as observed by endogenous alkaline phosphatase (EAP) staining assay. An in vitro transwell experiment revealed that the polypeptide inhibited vascular endothelial growth factor (VEGF) induced migration and tubulogenesis of human umbilical vein endothelial cells (HUVECs). Exposure of HUVECs in 20 mu g/ml PG155 significantly decreased the density of migrated cells. Almost complete inhibition of cell migration was found when HUVECs were treated with 40-80 mu g/ml PG155. PG155 (20 mu g/ml) markedly inhibited the tube formation of HUVECs and a dose-dependent effect was also found when treatment of HUVECs with PG155 at the concentration from 20 to 160 mu g/ml.; Using guanidine-HCl extraction, acetone precipitation, ultra-filtration and chromatography, a novel polypeptide with potent anti-angiogenic activity was purified from cartilage of the shark, Prionace glauca. N-terminal amino acid sequence analysis and SDS-PAGE revealed that the substance is a novel polypeptide with MW 15500 (PG155). The anti-angiogenic effects of PG155 were evaluated using zebrafish embryos model in vivo. Treatment of the embryos with 20 mu g/ml PG155 resulted in a significant reduction in the growth of subintestinal vessels (SIVs). A higher dose resulted in almost complete inhibition of SIV growth, as observed by endogenous alkaline phosphatase (EAP) staining assay. An in vitro transwell experiment revealed that the polypeptide inhibited vascular endothelial growth factor (VEGF) induced migration and tubulogenesis of human umbilical vein endothelial cells (HUVECs). Exposure of HUVECs in 20 mu g/ml PG155 significantly decreased the density of migrated cells. Almost complete inhibition of cell migration was found when HUVECs were treated with 40-80 mu g/ml PG155. PG155 (20 mu g/ml) markedly inhibited the tube formation of HUVECs and a dose-dependent effect was also found when treatment of HUVECs with PG155 at the concentration from 20 to 160 mu g/ml.
关键词	Anti-angiogenic Activity Isolation And Purification N-terminal Sequence Shark Cartilage Polypeptide Anti-cancer
学科领域	Oncology
收录类别	SCI
语种	英语
WOS记录号	WOS:000248259900034
引用统计	被引频次：38[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/5764
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China 3.Biopharmaceut Inst Shandong Prov, Jinan, Peoples R China
推荐引用方式 GB/T 7714	Zheng, Lanhong,Ling, Peixue,Wang, Zheng,et al. A novel polypeptide from shark cartilage with potent anti-angiogenic activity[J]. CANCER BIOLOGY & THERAPY,2007,6(5):775-780.
APA	Zheng, Lanhong.,Ling, Peixue.,Wang, Zheng.,Niu, Rongli.,Hu, Chaoxin.,...&Lin, Xiukun.(2007).A novel polypeptide from shark cartilage with potent anti-angiogenic activity.CANCER BIOLOGY & THERAPY,6(5),775-780.
MLA	Zheng, Lanhong,et al."A novel polypeptide from shark cartilage with potent anti-angiogenic activity".CANCER BIOLOGY & THERAPY 6.5(2007):775-780.

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