N-acetylchitooligosaccharide is a potent angiogenic inhibitor both in vivo and in vitro

doi:10.1016/j.bbrc.2007.03.094

IOCAS-IR > 实验海洋生物学重点实验室

	N-acetylchitooligosaccharide is a potent angiogenic inhibitor both in vivo and in vitro
	Wang, Zheng ; Zheng, Lanhong ; Yang, Shaoli ; Niu, Rongli ; Chu, Edward ; Lin, Xiukun
	2007-05-25
发表期刊	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN	0006-291X
卷号	357 期号:1 页码:26-31
文章类型	Article
摘要	N-Acetylchitooligosaccharide (N-acetyl-COs) was prepared by N-acetylation of chitooligosaccharide (COs). In vitro study using human umbilical vein endothelial cells (HUVECs) revealed that both N-acetyl-COs and COs inhibited the proliferation of HUVECs by inducing apoptosis. Treatment of HUVECs by N-acetyl-COs resulted in a significant reduction of density of the migration cells and repressed tubulogenesis process. The antiangiogenic effects of the oligosaccharides were further evaluated using in vivo zebrafish angiogenesis model, and the results showed that both oligosaccharides inhibited the growth of subintestinal vessels (SIV) of zebrafish embryos in a dose-dependent manner, as observed by endogenous alkaline phosphatase (EAP) staining assay. In contrast, no cytotoxicity was found when treating the NIH3T3 and several other cancer cells with the oligosaccharides. Our results also confirmed the antiangiogenic activity of N-acetyl-COs was significantly stronger than the parent oligosaccharide, COs. (c) 2007 Published by Elsevier Inc.; N-Acetylchitooligosaccharide (N-acetyl-COs) was prepared by N-acetylation of chitooligosaccharide (COs). In vitro study using human umbilical vein endothelial cells (HUVECs) revealed that both N-acetyl-COs and COs inhibited the proliferation of HUVECs by inducing apoptosis. Treatment of HUVECs by N-acetyl-COs resulted in a significant reduction of density of the migration cells and repressed tubulogenesis process. The antiangiogenic effects of the oligosaccharides were further evaluated using in vivo zebrafish angiogenesis model, and the results showed that both oligosaccharides inhibited the growth of subintestinal vessels (SIV) of zebrafish embryos in a dose-dependent manner, as observed by endogenous alkaline phosphatase (EAP) staining assay. In contrast, no cytotoxicity was found when treating the NIH3T3 and several other cancer cells with the oligosaccharides. Our results also confirmed the antiangiogenic activity of N-acetyl-COs was significantly stronger than the parent oligosaccharide, COs. (c) 2007 Published by Elsevier Inc.
关键词	N-acetylchitooligosaccharide Chitooligosaccharide Human Umbilical Vein Endothelial Cells Zebrafish Antiangiogenesis
学科领域	Biochemistry & Molecular Biology ; Biophysics
DOI	10.1016/j.bbrc.2007.03.094
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000246028800005
引用统计	被引频次：66[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/5678
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Yale Univ, Sch Med, Ctr Canc, New Haven, CT 06511 USA
推荐引用方式 GB/T 7714	Wang, Zheng,Zheng, Lanhong,Yang, Shaoli,et al. N-acetylchitooligosaccharide is a potent angiogenic inhibitor both in vivo and in vitro[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2007,357(1):26-31.
APA	Wang, Zheng,Zheng, Lanhong,Yang, Shaoli,Niu, Rongli,Chu, Edward,&Lin, Xiukun.(2007).N-acetylchitooligosaccharide is a potent angiogenic inhibitor both in vivo and in vitro.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,357(1),26-31.
MLA	Wang, Zheng,et al."N-acetylchitooligosaccharide is a potent angiogenic inhibitor both in vivo and in vitro".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 357.1(2007):26-31.

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