Substrate induction and statistical optimization for the production of chitosanase from Microbacterium sp OU01

doi:10.1016/j.biortech.2006.07.020

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	Substrate induction and statistical optimization for the production of chitosanase from Microbacterium sp OU01
	Sun, Yuying ; Han, Baoqin ; Liu, Wanshun ; Zhang, Jiquan ; Gao, Xingshuang
	2007-05-01
发表期刊	BIORESOURCE TECHNOLOGY
ISSN	0960-8524
卷号	98 期号:8 页码:1548-1553
文章类型	Article
摘要	The chitosanase production was markedly enhanced by substrate induction, statistical optimization of medium composition and culture conditions by Microbacteritan sp. OU01 in shake-flask. A significant influence of (NH(4))(2)SO(4), MgSO(4)center dot 7H(2)O and initial pH on chitosanase production was noted with Plackett-Burman design. It was then revealed with the method of steepest ascent and response surface methodology (RSM) that 19.0 g/L (NH(4))(2)SO(4), 1.3 g/L MgSO(4) and an initial pH of 2.0 were optimum for the production of chitosanase; colloidal chitosan appeared to be the best inducer for chitosanase production by Microbacterium sp. OU01. This optimization strategy led to the enhancement of chitosanase from 3.6 U/mL to 118 U/mL. (c) 2006 Elsevier Ltd. All rights reserved.; The chitosanase production was markedly enhanced by substrate induction, statistical optimization of medium composition and culture conditions by Microbacteritan sp. OU01 in shake-flask. A significant influence of (NH4)(2)SO4, MgSO4 center dot 7H(2)O and initial pH on chitosanase production was noted with Plackett-Burman design. It was then revealed with the method of steepest ascent and response surface methodology (RSM) that 19.0 g/L (NH4)(2)SO4, 1.3 g/L MgSO4 and an initial pH of 2.0 were optimum for the production of chitosanase; colloidal chitosan appeared to be the best inducer for chitosanase production by Microbacterium sp. OU01. This optimization strategy led to the enhancement of chitosanase from 3.6 U/mL to 118 U/mL. (c) 2006 Elsevier Ltd. All rights reserved.
关键词	Chitosanase Induction Colloidal Chitosan Statistical Optimization Plackett-burman Response Surface Methodology
学科领域	Agricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels
DOI	10.1016/j.biortech.2006.07.020
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000244775100006
引用统计	被引频次：36[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/5648
专题	实验海洋生物学重点实验室
作者单位	1.Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Sun, Yuying,Han, Baoqin,Liu, Wanshun,et al. Substrate induction and statistical optimization for the production of chitosanase from Microbacterium sp OU01[J]. BIORESOURCE TECHNOLOGY,2007,98(8):1548-1553.
APA	Sun, Yuying,Han, Baoqin,Liu, Wanshun,Zhang, Jiquan,&Gao, Xingshuang.(2007).Substrate induction and statistical optimization for the production of chitosanase from Microbacterium sp OU01.BIORESOURCE TECHNOLOGY,98(8),1548-1553.
MLA	Sun, Yuying,et al."Substrate induction and statistical optimization for the production of chitosanase from Microbacterium sp OU01".BIORESOURCE TECHNOLOGY 98.8(2007):1548-1553.

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