Molecular identification of 16 Porphyra lines using sequence-related amplified polymorphism markers

doi:10.1016/j.aquabot.2007.06.006

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	Molecular identification of 16 Porphyra lines using sequence-related amplified polymorphism markers
	Qiao, Li-Xian ; Liu, Hong-Yu ; Guo, Bao-Tai ; Weng, Man-Li ; Dai, Ji-Xun ; Duan, De-Lin ; Wang, Bin
	2007-10-01
发表期刊	AQUATIC BOTANY
ISSN	0304-3770
卷号	87 期号:3 页码:203-208
文章类型	Article
摘要	Sequence-related amplified polymorphism (SRAP) is a novel molecular marker technique designed to amplify open reading frames (ORFs). The SRAP analytic system was set up and applied to Porphyra germplasm identification in this study for the first time. Sixteen Porphyra lines were screened by SRAP technique with 30 primer combinations. In the analysis, 14 primer combinations produced stable and reproducible amplification patterns in three repetitive experiments. Among the total 533 amplified fragments, 522 (98%) were polymorphic, with an average of 38 fragments for each primer combination, ranging in size from 50 to 500 bp. The 533 fragments were visually scored one by one and then used to develop a dendrogram with Unweighted Pair-Group Method Arithmetic Average (UPGMA), and the 16 Porphyra lines were divided into two major groups at the 0.68 similarity level. From the total 533 fragments, I I amplified by two primer combinations, ME1/EM1 and ME4/EM6, were used to develop the DNA fingerprints of the 16 Porphyra lines. The DNA fingerprints were then converted into binary codes, with I and 0 representing presence and absence of the corresponding amplified fragment, respectively. In the DNA fingerprints, each of the 16 Porphyra lines has its unique binary code and can be easily distinguished from the others. This is the first report on the development of SRAP technique and its utilization in germplasm identification of seaweeds. The results demonstrated that SRAP is a simple, stable, polymorphic and reproducible molecular marker technique for the classification and identification of Porphyra lines. (c) 2007 Elsevier B.V. All rights reserved.; Sequence-related amplified polymorphism (SRAP) is a novel molecular marker technique designed to amplify open reading frames (ORFs). The SRAP analytic system was set up and applied to Porphyra germplasm identification in this study for the first time. Sixteen Porphyra lines were screened by SRAP technique with 30 primer combinations. In the analysis, 14 primer combinations produced stable and reproducible amplification patterns in three repetitive experiments. Among the total 533 amplified fragments, 522 (98%) were polymorphic, with an average of 38 fragments for each primer combination, ranging in size from 50 to 500 bp. The 533 fragments were visually scored one by one and then used to develop a dendrogram with Unweighted Pair-Group Method Arithmetic Average (UPGMA), and the 16 Porphyra lines were divided into two major groups at the 0.68 similarity level. From the total 533 fragments, I I amplified by two primer combinations, ME1/EM1 and ME4/EM6, were used to develop the DNA fingerprints of the 16 Porphyra lines. The DNA fingerprints were then converted into binary codes, with I and 0 representing presence and absence of the corresponding amplified fragment, respectively. In the DNA fingerprints, each of the 16 Porphyra lines has its unique binary code and can be easily distinguished from the others. This is the first report on the development of SRAP technique and its utilization in germplasm identification of seaweeds. The results demonstrated that SRAP is a simple, stable, polymorphic and reproducible molecular marker technique for the classification and identification of Porphyra lines. (c) 2007 Elsevier B.V. All rights reserved.
关键词	Molecular Identification Porphyra Sequence-related Amplified Polymorphism
学科领域	Plant Sciences ; Marine & Freshwater Biology
DOI	10.1016/j.aquabot.2007.06.006
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000249874900004
引用统计	被引频次：21[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/5634
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Plant Genom, Beijing 100101, Peoples R China 2.Ocean Univ China, Dept Life Sci & Technol, Qingdao 266003, Peoples R China 3.Qingdao Agr Univ, Coll Life Sci, Qingdao 266109, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Qiao, Li-Xian,Liu, Hong-Yu,Guo, Bao-Tai,et al. Molecular identification of 16 Porphyra lines using sequence-related amplified polymorphism markers[J]. AQUATIC BOTANY,2007,87(3):203-208.
APA	Qiao, Li-Xian.,Liu, Hong-Yu.,Guo, Bao-Tai.,Weng, Man-Li.,Dai, Ji-Xun.,...&Wang, Bin.(2007).Molecular identification of 16 Porphyra lines using sequence-related amplified polymorphism markers.AQUATIC BOTANY,87(3),203-208.
MLA	Qiao, Li-Xian,et al."Molecular identification of 16 Porphyra lines using sequence-related amplified polymorphism markers".AQUATIC BOTANY 87.3(2007):203-208.

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