Molecular characteristics and expression analysis of calreticulin in Chinese shrimp Fenneropenaeus chinensis

doi:10.1016/j.cbpb.2007.03.001

IOCAS-IR > 实验海洋生物学重点实验室

	Molecular characteristics and expression analysis of calreticulin in Chinese shrimp Fenneropenaeus chinensis
	Luana, Wei ; Li, Fuhua ; Wang, Bing ; Zhang, Xiaojun ; Liu, Yichen ; Xiang, Jianhai
	2007-07-01
发表期刊	COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
ISSN	1096-4959
卷号	147 期号:3 页码:482-491
文章类型	Article
摘要	Calreticulin (CRT), as an endoplasmic reticulum luminal resident protein, plays important roles in Ca2+ homeostasis and molecular chaperoning. CRT on the surface of the cell can modulate cell adhesion, phagocytosis and integrin-dependent Ca2+ signaling. The full length cDNA of calreticulin (FcCRT) was cloned from Chinese shrimp Fenneropenaeus chinensis. It consists of 1672 by with an open reading frame of 1221 bp, encoding 406 amino acids. This is the first reported cDNA sequence of calreticulin in Crustacea. The deduced amino acid sequence of FcCRT showed high identity with those of Bombyx mori (88%), Drosophila melanogaster (83%), Mus musculus (82%) and Homo sapiens (82%). Highest expression of FcCRT was detected in ovary by Northern blot and in situ hybridization. Different mRNA levels of FcCRT were detected at various molting stages. Expression of FcCRT was induced significantly after 3 h of heat shock treatment, reached the maximum at 4 h and dropped after that. Differential expression profiles of FcCRT were observed in hepatopancreas and haemocytes when shrimp were challenged by white spot syndrome virus (WSSV). From the above results, we inferred that FcCRT might play important roles in Ca2+ homeostasis, chaperoning and immune function in shrimp. (c) 2007 Elsevier Inc. All rights reserved.; Calreticulin (CRT), as an endoplasmic reticulum luminal resident protein, plays important roles in Ca2+ homeostasis and molecular chaperoning. CRT on the surface of the cell can modulate cell adhesion, phagocytosis and integrin-dependent Ca2+ signaling. The full length cDNA of calreticulin (FcCRT) was cloned from Chinese shrimp Fenneropenaeus chinensis. It consists of 1672 by with an open reading frame of 1221 bp, encoding 406 amino acids. This is the first reported cDNA sequence of calreticulin in Crustacea. The deduced amino acid sequence of FcCRT showed high identity with those of Bombyx mori (88%), Drosophila melanogaster (83%), Mus musculus (82%) and Homo sapiens (82%). Highest expression of FcCRT was detected in ovary by Northern blot and in situ hybridization. Different mRNA levels of FcCRT were detected at various molting stages. Expression of FcCRT was induced significantly after 3 h of heat shock treatment, reached the maximum at 4 h and dropped after that. Differential expression profiles of FcCRT were observed in hepatopancreas and haemocytes when shrimp were challenged by white spot syndrome virus (WSSV). From the above results, we inferred that FcCRT might play important roles in Ca2+ homeostasis, chaperoning and immune function in shrimp. (c) 2007 Elsevier Inc. All rights reserved.
关键词	Calreticulin Cloning Expression Fenneropenaeus Chinensis Heat Shock Molting Stage Wssv Challenge
学科领域	Biochemistry & Molecular Biology ; Zoology
DOI	10.1016/j.cbpb.2007.03.001
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000247555900016
引用统计	被引频次：48[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/5610
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Luana, Wei,Li, Fuhua,Wang, Bing,et al. Molecular characteristics and expression analysis of calreticulin in Chinese shrimp Fenneropenaeus chinensis[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,2007,147(3):482-491.
APA	Luana, Wei,Li, Fuhua,Wang, Bing,Zhang, Xiaojun,Liu, Yichen,&Xiang, Jianhai.(2007).Molecular characteristics and expression analysis of calreticulin in Chinese shrimp Fenneropenaeus chinensis.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,147(3),482-491.
MLA	Luana, Wei,et al."Molecular characteristics and expression analysis of calreticulin in Chinese shrimp Fenneropenaeus chinensis".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 147.3(2007):482-491.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
luana-Molecular char（1697KB）			限制开放	--	浏览