Molecular coordinated regulation of gene expression during ovarian development in the penaeid shrimp

doi:10.1007/s10126-007-9006-4

IOCAS-IR > 实验海洋生物学重点实验室

	Molecular coordinated regulation of gene expression during ovarian development in the penaeid shrimp
	Lo, Ting Sze ; Cui, Zhaoxia ; Mong, Janice L. Y.; Wong, Queenie W. L.; Chan, Siu-Ming ; Kwan, Hoi Shan ; Chu, Ka Hou
	2007-08-01
发表期刊	MARINE BIOTECHNOLOGY
ISSN	1436-2228
卷号	9 期号:4 页码:459-468
文章类型	Article
摘要	To understand the molecular events of ovarian development in penaeid shrimp, RNA arbitrarily primed polymerase chain reaction (RAP-PCR) was used to identify differentially expressed genes during ovarian maturation in Metapenaeus ensis. From a screening of 700 clones in a cDNA library of the shrimp ovary by the products of RAP-PCR of different maturation stages, 91 fragments with differentially expressed pattern as revealed by dot-blot hybridization were isolated and sequenced. Forty-two of these fragments show significant sequence similarity to known gene products and the differentially expressed pattern of 10 putative genes were further characterized via Northern hybridization. Putative glyceraldehyde-3-phosphate dehydrogenase and arginine kinase are related to provision of energy for active cellular function in oocyte development. Translationally controlled tumor protein, actin, and keratin are related to the organization of cytoskeleton to accomplish growth and development of oocytes. High mobility group protein DSP1, heat shock protein 70, and nucleoside diphosphate kinase may act as repressors before the onset of ovarian maturation. Peptidyl-prolyl cis-trans isomerase and glutathione peroxidase are related to the stabilization of proteins and oocytes. This study provides new insights on the molecular events in the ovarian development in the shrimp.; To understand the molecular events of ovarian development in penaeid shrimp, RNA arbitrarily primed polymerase chain reaction (RAP-PCR) was used to identify differentially expressed genes during ovarian maturation in Metapenaeus ensis. From a screening of 700 clones in a cDNA library of the shrimp ovary by the products of RAP-PCR of different maturation stages, 91 fragments with differentially expressed pattern as revealed by dot-blot hybridization were isolated and sequenced. Forty-two of these fragments show significant sequence similarity to known gene products and the differentially expressed pattern of 10 putative genes were further characterized via Northern hybridization. Putative glyceraldehyde-3-phosphate dehydrogenase and arginine kinase are related to provision of energy for active cellular function in oocyte development. Translationally controlled tumor protein, actin, and keratin are related to the organization of cytoskeleton to accomplish growth and development of oocytes. High mobility group protein DSP1, heat shock protein 70, and nucleoside diphosphate kinase may act as repressors before the onset of ovarian maturation. Peptidyl-prolyl cis-trans isomerase and glutathione peroxidase are related to the stabilization of proteins and oocytes. This study provides new insights on the molecular events in the ovarian development in the shrimp.
关键词	Gene Expression Metapenaeus Ensis Ovarian Development Penaeidae
学科领域	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
DOI	10.1007/s10126-007-9006-4
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000249731400007
引用统计	被引频次：28[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/5608
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Univ Hong Kong, Dept Biochem, Shatin, Peoples R China 2.Chinese Univ Hong Kong, Dept Anat & Cellular Pathol, Shatin, Hong Kong, Peoples R China 3.Chinese Univ Hong Kong, Dept Biol, Shatin, Hong Kong, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China 5.Univ Hong Kong, Dept Zool, Hong Kong, Peoples R China
推荐引用方式 GB/T 7714	Lo, Ting Sze,Cui, Zhaoxia,Mong, Janice L. Y.,et al. Molecular coordinated regulation of gene expression during ovarian development in the penaeid shrimp[J]. MARINE BIOTECHNOLOGY,2007,9(4):459-468.
APA	Lo, Ting Sze.,Cui, Zhaoxia.,Mong, Janice L. Y..,Wong, Queenie W. L..,Chan, Siu-Ming.,...&Chu, Ka Hou.(2007).Molecular coordinated regulation of gene expression during ovarian development in the penaeid shrimp.MARINE BIOTECHNOLOGY,9(4),459-468.
MLA	Lo, Ting Sze,et al."Molecular coordinated regulation of gene expression during ovarian development in the penaeid shrimp".MARINE BIOTECHNOLOGY 9.4(2007):459-468.

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