Isolation and characterization of photosystem II from the filamentous sporophyte of Porphyra yezoensis

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	Isolation and characterization of photosystem II from the filamentous sporophyte of Porphyra yezoensis
	Gao, Z. Q.2; Meng, C. X.2; Wang, G. C.1; Ye, N. H.3
	2008-08-01
发表期刊	INDIAN JOURNAL OF BIOCHEMISTRY & BIOPHYSICS
ISSN	0301-1208
卷号	45 期号:4 页码:244-249
文章类型	Article
摘要	Thylakoid membranes were isolated and purified from diploid filamentous sporophytes of Porphyra yezoensis Ueda using sucrose density gradient ultracentrifugation (SDGUC). After thylakoid membranes were solubilized with SDS, the photosystem II (PSII) particles with high 2, 6-dichloroindophenol (DCIP) photoreduction activity were isolated by SDGUC. The absorption and fluorescence spectra, DCIP photoreduction activity and oxygen evolution activity of the thylakoid membranes and PSII particles were determined. The polypeptide composition of purified PSII particles was distinguished by SDS-PAGE. Results showed that PSII particles of sporophytes differed from the gametophytes in spectral properties and polypeptide composition. Apart from 55 kDa D1-D2 heterodimer, CP47, CP43, 33 kDa protein was also detected. However, cyt c-550, 20 kDa, 14 kDa and 16 kDa proteins found in PSII particles from gametophytes were not detected in the sporophytes.; Thylakoid membranes were isolated and purified from diploid filamentous sporophytes of Porphyra yezoensis Ueda using sucrose density gradient ultracentrifugation (SDGUC). After thylakoid membranes were solubilized with SDS, the photosystem II (PSII) particles with high 2, 6-dichloroindophenol (DCIP) photoreduction activity were isolated by SDGUC. The absorption and fluorescence spectra, DCIP photoreduction activity and oxygen evolution activity of the thylakoid membranes and PSII particles were determined. The polypeptide composition of purified PSII particles was distinguished by SDS-PAGE. Results showed that PSII particles of sporophytes differed from the gametophytes in spectral properties and polypeptide composition. Apart from 55 kDa D1-D2 heterodimer, CP47, CP43, 33 kDa protein was also detected. However, cyt c-550, 20 kDa, 14 kDa and 16 kDa proteins found in PSII particles from gametophytes were not detected in the sporophytes.
关键词	Porphyra Yezoensis Photosytem Ii Filamentous Sporophytes Thylakoid Membranes Polypeptide Composition Sucrose Density Gradient Ultracentrifugation
学科领域	Biochemistry & Molecular Biology ; Biophysics
收录类别	SCI
语种	英语
WOS记录号	WOS:000259435400004
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/5556
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Shandong Univ Technol, Sch Life Sci, Zibo 255049, Peoples R China 3.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Gao, Z. Q.,Meng, C. X.,Wang, G. C.,et al. Isolation and characterization of photosystem II from the filamentous sporophyte of Porphyra yezoensis[J]. INDIAN JOURNAL OF BIOCHEMISTRY & BIOPHYSICS,2008,45(4):244-249.
APA	Gao, Z. Q.,Meng, C. X.,Wang, G. C.,&Ye, N. H..(2008).Isolation and characterization of photosystem II from the filamentous sporophyte of Porphyra yezoensis.INDIAN JOURNAL OF BIOCHEMISTRY & BIOPHYSICS,45(4),244-249.
MLA	Gao, Z. Q.,et al."Isolation and characterization of photosystem II from the filamentous sporophyte of Porphyra yezoensis".INDIAN JOURNAL OF BIOCHEMISTRY & BIOPHYSICS 45.4(2008):244-249.

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