Fatty acid biosynthesis in eukaryotic photosynthetic microalgae: Identification of a microsomal delta 12 desaturase in Chlamydomonas reinhardtii

doi:10.1007/s12275-007-0223-3

IOCAS-IR > 实验海洋生物学重点实验室

	Fatty acid biosynthesis in eukaryotic photosynthetic microalgae: Identification of a microsomal delta 12 desaturase in Chlamydomonas reinhardtii
	Chi, Xiaoyuan 1,2; Zhang, Xiaowen 1,2; Guan, Xiangyu 3; Ding, Ling 1,2; Li, Youxun 1,2; Wang, Mingqing 3; Lin, Hanzhi 1,2; Qin, Song 1
	2008-04-01
发表期刊	JOURNAL OF MICROBIOLOGY
ISSN	1225-8873
卷号	46 期号:2 页码:189-201
文章类型	Article
摘要	Polyunsaturated fatty acids (PUFAs) are important components of infant and adult nutrition because they serve as structural elements of cell membranes. Fatty acid desaturases are responsible for the insertion of double bonds into pre-formed fatty acid chains in reactions that require oxygen and reducing equivalents. In this study, the genome-wide characterization of the fatty acid desaturases from seven eukaryotic photosynthetic microalgae was undertaken according to the conserved histidine-rich motifs and phylogenetic profiles. Analysis of these genomes provided insight into the origin and evolution of the pathway of fatty acid biosynthesis in eukaryotic plants. In addition, the candidate enzyme from Chlamydomonas reinhardtii with the highest similarity to the microsomal Delta 12 desaturase of Chlorella vulgaris was isolated, and its function was verified by heterologous expression in yeast (Saccharomyces cerevisiae).; Polyunsaturated fatty acids (PUFAs) are important components of infant and adult nutrition because they serve as structural elements of cell membranes. Fatty acid desaturases are responsible for the insertion of double bonds into pre-formed fatty acid chains in reactions that require oxygen and reducing equivalents. In this study, the genome-wide characterization of the fatty acid desaturases from seven eukaryotic photosynthetic microalgae was undertaken according to the conserved histidine-rich motifs and phylogenetic profiles. Analysis of these genomes provided insight into the origin and evolution of the pathway of fatty acid biosynthesis in eukaryotic plants. In addition, the candidate enzyme from Chlamydomonas reinhardtii with the highest similarity to the microsomal Delta 12 desaturase of Chlorella vulgaris was isolated, and its function was verified by heterologous expression in yeast (Saccharomyces cerevisiae).
关键词	Fatty Acid Desaturase Eukaryotic Photosynthetic Microalgae Chlamydomonas Reinhardtii
学科领域	Microbiology
DOI	10.1007/s12275-007-0223-3
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000255438700010
引用统计	被引频次：37[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/5526
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China 2.Grad Univ, Chinese Acad Sci, Beijing 100039, Peoples R China 3.Ocean Univ China, Coll Marine Life Sci, Fac Life Sci & Technol, Qingdao 266003, Peoples R China
推荐引用方式 GB/T 7714	Chi, Xiaoyuan,Zhang, Xiaowen,Guan, Xiangyu,et al. Fatty acid biosynthesis in eukaryotic photosynthetic microalgae: Identification of a microsomal delta 12 desaturase in Chlamydomonas reinhardtii[J]. JOURNAL OF MICROBIOLOGY,2008,46(2):189-201.
APA	Chi, Xiaoyuan.,Zhang, Xiaowen.,Guan, Xiangyu.,Ding, Ling.,Li, Youxun.,...&Qin, Song.(2008).Fatty acid biosynthesis in eukaryotic photosynthetic microalgae: Identification of a microsomal delta 12 desaturase in Chlamydomonas reinhardtii.JOURNAL OF MICROBIOLOGY,46(2),189-201.
MLA	Chi, Xiaoyuan,et al."Fatty acid biosynthesis in eukaryotic photosynthetic microalgae: Identification of a microsomal delta 12 desaturase in Chlamydomonas reinhardtii".JOURNAL OF MICROBIOLOGY 46.2(2008):189-201.

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