Structural analysis of SNARE motifs from sea perch, Lateolabrax japonicus by computerized approaches

doi:10.1016/j.compbiolchem.2007.08-002

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	Structural analysis of SNARE motifs from sea perch, Lateolabrax japonicus by computerized approaches
	Chen, Kui ; Huang, Xiaohang
	2007-10-01
发表期刊	COMPUTATIONAL BIOLOGY AND CHEMISTRY
ISSN	1476-9271
卷号	31 期号:5-6 页码:378-383
文章类型	Article
摘要	Three cDNA sequences encoding four SNARE (N-ethylmaleimide-sensitive fusion protein attachment protein receptors) motifs were cloned from sea perch, and the deduced peptide sequences were analyzed for structural prediction by using 14 different web servers and softwares. The "ionic layer" structure, the three dimensional extension and conformational characters of the SNARE 7S core complex by using bioinformatics approaches were compared respectively with those from mammalian X-ray crystallographic investigations. The result suggested that the formation and stabilization of fish SNARE core complex might be driven by hydrophobic association, hydrogen bond among R group of core amino acids and electrostatic attraction at molecular level. This revealed that the SNARE proteins interaction of the fish may share the same molecular mechanism with that of mammal, indicating the universality and solidity of SNARE core complex theory. This work is also an attempt to get the protein 3D structural information which appears to be similar to that obtained through X-ray crystallography, only by using computerized approaches. (C) 2007 Elsevier Ltd. All rights reserved.; Three cDNA sequences encoding four SNARE (N-ethylmaleimide-sensitive fusion protein attachment protein receptors) motifs were cloned from sea perch, and the deduced peptide sequences were analyzed for structural prediction by using 14 different web servers and softwares. The "ionic layer" structure, the three dimensional extension and conformational characters of the SNARE 7S core complex by using bioinformatics approaches were compared respectively with those from mammalian X-ray crystallographic investigations. The result suggested that the formation and stabilization of fish SNARE core complex might be driven by hydrophobic association, hydrogen bond among R group of core amino acids and electrostatic attraction at molecular level. This revealed that the SNARE proteins interaction of the fish may share the same molecular mechanism with that of mammal, indicating the universality and solidity of SNARE core complex theory. This work is also an attempt to get the protein 3D structural information which appears to be similar to that obtained through X-ray crystallography, only by using computerized approaches. (C) 2007 Elsevier Ltd. All rights reserved.
关键词	Sea Perch Snare Proteins Motif 7s Core Complex Protein Structure Analysis
学科领域	Biology ; Computer Science, Interdisciplinary Applications
DOI	10.1016/j.compbiolchem.2007.08-002
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000250912400009
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/5520
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.State Ocean Adm, Inst Oceanog 1, Qingdao 266061, Peoples R China 3.Grad Univ Chinese Acad Sci, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Chen, Kui,Huang, Xiaohang. Structural analysis of SNARE motifs from sea perch, Lateolabrax japonicus by computerized approaches[J]. COMPUTATIONAL BIOLOGY AND CHEMISTRY,2007,31(5-6):378-383.
APA	Chen, Kui,&Huang, Xiaohang.(2007).Structural analysis of SNARE motifs from sea perch, Lateolabrax japonicus by computerized approaches.COMPUTATIONAL BIOLOGY AND CHEMISTRY,31(5-6),378-383.
MLA	Chen, Kui,et al."Structural analysis of SNARE motifs from sea perch, Lateolabrax japonicus by computerized approaches".COMPUTATIONAL BIOLOGY AND CHEMISTRY 31.5-6(2007):378-383.

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