Antioxidant activity and cytoprotective effect of kappa-carrageenan oligosaccharides and their different derivatives

doi:10.1016/j.bmcl.2005.11.057

	Antioxidant activity and cytoprotective effect of kappa-carrageenan oligosaccharides and their different derivatives
	Yuan, HM; Song, JM; Zhang, WW ; Li, XG ; Li, N ; Gao, XL
	2006-03-01
发表期刊	BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
ISSN	0960-894X
卷号	16 期号:5 页码:1329-1334
文章类型	Article
摘要	Antioxidant activity of kappa-carrageenan oligosaccharides (OM) and their chemical modification derivatives was investigated employing various established in vitro systems, such as reducing power, iron ion chelation, and total antioxidant activity using beta-carotene-linoleic acid system. The oversulfated (SD), lowly (LAD), and highly acetylated derivatives (HAD) in reducing power assay, the phosphorylated derivative (PD) in metal chelating assay, and oversulfated and phosphorylated derivatives in total antioxidant activity assay exhibited antioxidant activity higher than that of carrageenan oligosaccharides. The results indicated that the chemical modification of carrageenan oligosaccharides can enhance their antioxidant activity in vitro. The protective effects of the carrageenan oligosaccharides and their chemically modified derivatives against H2O2 and UVA (long-wave ultraviolet radiation) induced oxidative damage on rat thymic lymphocyte were investigated by measuring cell viability via 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT). Thymic lymphocyte exposure to H2O2 and UVA, a marked reduction in cell survival was observed, which was significantly prevented by carrageenan oligosaccharides and their derivatives (preincubated for 2 h) at 66.7-2000 mu g/mL. But both the carrageenan oligosaccharides and their different derivatives showed the similar protective effects on intracellular level. Taken together, these results suggest that carrageenan oligosaccharides and their derivatives show relevant antioxidant activity both in vitro and in a cell system. (C) 2005 Elsevier Ltd. All rights reserved.; Antioxidant activity of kappa-carrageenan oligosaccharides (OM) and their chemical modification derivatives was investigated employing various established in vitro systems, such as reducing power, iron ion chelation, and total antioxidant activity using beta-carotene-linoleic acid system. The oversulfated (SD), lowly (LAD), and highly acetylated derivatives (HAD) in reducing power assay, the phosphorylated derivative (PD) in metal chelating assay, and oversulfated and phosphorylated derivatives in total antioxidant activity assay exhibited antioxidant activity higher than that of carrageenan oligosaccharides. The results indicated that the chemical modification of carrageenan oligosaccharides can enhance their antioxidant activity in vitro. The protective effects of the carrageenan oligosaccharides and their chemically modified derivatives against H2O2 and UVA (long-wave ultraviolet radiation) induced oxidative damage on rat thymic lymphocyte were investigated by measuring cell viability via 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT). Thymic lymphocyte exposure to H2O2 and UVA, a marked reduction in cell survival was observed, which was significantly prevented by carrageenan oligosaccharides and their derivatives (preincubated for 2 h) at 66.7-2000 mu g/mL. But both the carrageenan oligosaccharides and their different derivatives showed the similar protective effects on intracellular level. Taken together, these results suggest that carrageenan oligosaccharides and their derivatives show relevant antioxidant activity both in vitro and in a cell system. (C) 2005 Elsevier Ltd. All rights reserved.
关键词	Carrageeenan Oligosaccharides Chemically Modified Derivatives Antioxidant Hydrogen Peroxide Uva
学科领域	Chemistry, Medicinal ; Chemistry, Organic
DOI	10.1016/j.bmcl.2005.11.057
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000235284700048
引用统计	被引频次：77[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/5486
专题	海洋生态与环境科学重点实验室海洋地质与环境重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Yuan, HM,Song, JM,Zhang, WW,et al. Antioxidant activity and cytoprotective effect of kappa-carrageenan oligosaccharides and their different derivatives[J]. BIOORGANIC & MEDICINAL CHEMISTRY LETTERS,2006,16(5):1329-1334.
APA	Yuan, HM,Song, JM,Zhang, WW,Li, XG,Li, N,&Gao, XL.(2006).Antioxidant activity and cytoprotective effect of kappa-carrageenan oligosaccharides and their different derivatives.BIOORGANIC & MEDICINAL CHEMISTRY LETTERS,16(5),1329-1334.
MLA	Yuan, HM,et al."Antioxidant activity and cytoprotective effect of kappa-carrageenan oligosaccharides and their different derivatives".BIOORGANIC & MEDICINAL CHEMISTRY LETTERS 16.5(2006):1329-1334.

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