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Protein phosphatase inhibition assay for detection of diarrhetic shellfish poison in oyster
Li, AF; Yu, RC; Li, J; Tang, XH; Wang, YF; Yan, T; Zhou, MJ
2006-03-01
发表期刊CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
ISSN0253-3820
卷号34期号:3页码:283-287
文章类型Article
摘要A method based on protein phosphatase enzyme activity inhibition for the detection of diarrhetic shellfish poison (DSP) was used to analyze the DSP toxicity in three oyster samples. Based on the standard dose-effect curve developed with a series of okadaic acid (OA) standard solutions, the DSP toxicity of the three oyster samples collected were screened, and the results showed that there were no OA and dinophysis toxins ( DTXs) in the samples without hydrolization. However, the OA toxicity could be detected in two of the hydrolyzed samples, and the OA toxicity of the two samples were 1.81 and 1.21 mu g OA eq./kg oyster, respectively.; A method based on protein phosphatase enzyme activity inhibition for the detection of diarrhetic shellfish poison (DSP) was used to analyze the DSP toxicity in three oyster samples. Based on the standard dose-effect curve developed with a series of okadaic acid (OA) standard solutions, the DSP toxicity of the three oyster samples collected were screened, and the results showed that there were no OA and dinophysis toxins ( DTXs) in the samples without hydrolization. However, the OA toxicity could be detected in two of the hydrolyzed samples, and the OA toxicity of the two samples were 1.81 and 1.21 mu g OA eq./kg oyster, respectively.
关键词Protein Phosphatase Diarrhetic Shellfish Poison Okadaic Acid Oyster
学科领域Chemistry, Analytical
收录类别SCI
语种英语
WOS记录号WOS:000236528300001
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/5438
专题海洋生态与环境科学重点实验室
海洋生物技术研发中心
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
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GB/T 7714
Li, AF,Yu, RC,Li, J,et al. Protein phosphatase inhibition assay for detection of diarrhetic shellfish poison in oyster[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2006,34(3):283-287.
APA Li, AF.,Yu, RC.,Li, J.,Tang, XH.,Wang, YF.,...&Zhou, MJ.(2006).Protein phosphatase inhibition assay for detection of diarrhetic shellfish poison in oyster.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,34(3),283-287.
MLA Li, AF,et al."Protein phosphatase inhibition assay for detection of diarrhetic shellfish poison in oyster".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 34.3(2006):283-287.
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