Toxin-screening and identification of bacteria isolated from highly toxic marine gastropod Nassarius semiplicatus

doi:10.1016/j.toxicon.2008.04.170

	Toxin-screening and identification of bacteria isolated from highly toxic marine gastropod Nassarius semiplicatus
	Wang, Xiao-Jie 1,2; Yu, Ren-Cheng 1; Luo, Xuan 1; Zhou, Ming-Jiang 1; Lin, Xiang-Tian 3
	2008-07-01
发表期刊	TOXICON
ISSN	0041-0101
卷号	52 期号:1 页码:55-61
文章类型	Article
摘要	Bacteria isolated from a highly toxic sample of gastropod Nassarius semiplicatus in Lianyungang, Jiangsu Province in July 2007, were studied to probe into the relationship between bacteria and toxicity of nassariid gastropod. The toxicity of the gastropod sample was 2 x 10(2) mouse unit (MU) Per gram Of tissue (wet weight). High concentration of tetrodotoxin (TTX) and its analogues (TTXs) were found in the digestive gland and muscle of the gastropod, using high performance liquid chromatography coupled with mass chromatography (LC-MS). Bacterial strains isolated from the digestive gland were cultured and screened for TTX with a competitive ELISA method. Tetrodotoxin was detected in a proportion of bacterial strains, but the toxin content was low. Partial 16S ribosomal DNA (rDNA) of the TTX-producing strains was then sequenced and compared with those published in the GenBank to tentatively identify the toxic strains. It was found that most of the toxic strains were closely affiliated with genus Vibrio, and the others were related to genus Shewanella, Marinomonas, Tenacibaculum and Aeromonas. These findings suggest that tetrodotoxin-producing bacteria might play an important role in tetrodotoxin accumulation/production in N. semiplicatus. (C) 2008 Elsevier Ltd. All rights reserved.; Bacteria isolated from a highly toxic sample of gastropod Nassarius semiplicatus in Lianyungang, Jiangsu Province in July 2007, were studied to probe into the relationship between bacteria and toxicity of nassariid gastropod. The toxicity of the gastropod sample was 2 x 10(2) mouse unit (MU) Per gram Of tissue (wet weight). High concentration of tetrodotoxin (TTX) and its analogues (TTXs) were found in the digestive gland and muscle of the gastropod, using high performance liquid chromatography coupled with mass chromatography (LC-MS). Bacterial strains isolated from the digestive gland were cultured and screened for TTX with a competitive ELISA method. Tetrodotoxin was detected in a proportion of bacterial strains, but the toxin content was low. Partial 16S ribosomal DNA (rDNA) of the TTX-producing strains was then sequenced and compared with those published in the GenBank to tentatively identify the toxic strains. It was found that most of the toxic strains were closely affiliated with genus Vibrio, and the others were related to genus Shewanella, Marinomonas, Tenacibaculum and Aeromonas. These findings suggest that tetrodotoxin-producing bacteria might play an important role in tetrodotoxin accumulation/production in N. semiplicatus. (C) 2008 Elsevier Ltd. All rights reserved.
关键词	Nassarius Semiplicatus Tetrodotoxin Bacteria Lc-ms Elisa 16s Rdna
学科领域	Pharmacology & Pharmacy ; Toxicology
DOI	10.1016/j.toxicon.2008.04.170
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000258816500007
引用统计	被引频次：67[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/5396
专题	海洋生态与环境科学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Hlth Inspect Agcy Lianyungang, Lianyungang 222006, Peoples R China
推荐引用方式 GB/T 7714	Wang, Xiao-Jie,Yu, Ren-Cheng,Luo, Xuan,et al. Toxin-screening and identification of bacteria isolated from highly toxic marine gastropod Nassarius semiplicatus[J]. TOXICON,2008,52(1):55-61.
APA	Wang, Xiao-Jie,Yu, Ren-Cheng,Luo, Xuan,Zhou, Ming-Jiang,&Lin, Xiang-Tian.(2008).Toxin-screening and identification of bacteria isolated from highly toxic marine gastropod Nassarius semiplicatus.TOXICON,52(1),55-61.
MLA	Wang, Xiao-Jie,et al."Toxin-screening and identification of bacteria isolated from highly toxic marine gastropod Nassarius semiplicatus".TOXICON 52.1(2008):55-61.

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