Investigation of Extration Method for Paralytic Shellfish Poisoning Toxins in Shellfish

	Investigation of Extration Method for Paralytic Shellfish Poisoning Toxins in Shellfish
	Jiang Tao1,2,3 ; Jiang Tian-Jiu 3,4
	2008-11-01
发表期刊	CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
ISSN	0253-3820
卷号	36 期号:11 页码:1460-1464
文章类型	Article
摘要	Me optimal conditions were established for the extraction of paralytic shellfish poisoning toxins from gonad of Chlamys nobills using acetic acid and hydrochloric acid in the concentration range of 0.04-1.0 mol/L. A 10-g portion of gonad of Chlamys nobilis was extracted by boiling for 5 min with 1.0 mL acetic acid and hydrochloric acid in a 50-mL beaker. Meanwhile, a portion of gonad of Chlamys nobilis was extracted by sonication in the solution of 0.3 mol/L HAc + 0.2 mol/L HCl for a total period of 5-30 min. The raw extract was centrifuged at 3500 r/min for 5 min and the pH of supernatant was adjusted from 2.0 to 4.0 by 0.1 mol/L NaOH or 5 mol/L HCL After passing through a Millipore ultrafiltration membrane (10000 MW cut-off), ultrafiltrate was then analyzed by HPLC. The results showed that hydrochloric acid in the concentration range of 0.25-1.0 mol/L caused a significant decrease of N-sulfocarbarnoyl-11-hydroxysulfate toxin C1 (C1), C2 and gonyautoxin 5 (GTX5) and the concomitant increase of GTX2,3. However, the amount of the three unstable toxins did not show any change using the extraction with acetic acid. Under the same concentration of acetic acid (0.3 mol/L) and hydrochloric acid (0.2 mol/L), the amount of C1 in the ultrasonic extraction was obviously lower than the boiling one, while C2 showed slightly higher than the latter.; Me optimal conditions were established for the extraction of paralytic shellfish poisoning toxins from gonad of Chlamys nobills using acetic acid and hydrochloric acid in the concentration range of 0.04-1.0 mol/L. A 10-g portion of gonad of Chlamys nobilis was extracted by boiling for 5 min with 1.0 mL acetic acid and hydrochloric acid in a 50-mL beaker. Meanwhile, a portion of gonad of Chlamys nobilis was extracted by sonication in the solution of 0.3 mol/L HAc + 0.2 mol/L HCl for a total period of 5-30 min. The raw extract was centrifuged at 3500 r/min for 5 min and the pH of supernatant was adjusted from 2.0 to 4.0 by 0.1 mol/L NaOH or 5 mol/L HCL After passing through a Millipore ultrafiltration membrane (10000 MW cut-off), ultrafiltrate was then analyzed by HPLC. The results showed that hydrochloric acid in the concentration range of 0.25-1.0 mol/L caused a significant decrease of N-sulfocarbarnoyl-11-hydroxysulfate toxin C1 (C1), C2 and gonyautoxin 5 (GTX5) and the concomitant increase of GTX2,3. However, the amount of the three unstable toxins did not show any change using the extraction with acetic acid. Under the same concentration of acetic acid (0.3 mol/L) and hydrochloric acid (0.2 mol/L), the amount of C1 in the ultrasonic extraction was obviously lower than the boiling one, while C2 showed slightly higher than the latter.
关键词	Paralytic Shellfish Poisoning Toxins High Performance Liquid Chromatography Extraction
学科领域	Chemistry, Analytical
收录类别	SCI
语种	英语
WOS记录号	WOS:000261465900002
引用统计	被引频次：4[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/5348
专题	海洋生态与环境科学重点实验室海洋地质与环境重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Jinan Univ, Dept Environm Engn, Guangzhou 510632, Guangdong, Peoples R China 4.S China Normal Univ, Coll Life Sci & Technol, Guangzhou 510632, Guangdong, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Jiang Tao,Jiang Tian-Jiu. Investigation of Extration Method for Paralytic Shellfish Poisoning Toxins in Shellfish[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2008,36(11):1460-1464.
APA	Jiang Tao,&Jiang Tian-Jiu.(2008).Investigation of Extration Method for Paralytic Shellfish Poisoning Toxins in Shellfish.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,36(11),1460-1464.
MLA	Jiang Tao,et al."Investigation of Extration Method for Paralytic Shellfish Poisoning Toxins in Shellfish".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 36.11(2008):1460-1464.

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