Selective and specific detection of sulfate-reducing bacteria using potentiometric stripping analysis

doi:10.1016/j.talanta.2010.07.030

	Selective and specific detection of sulfate-reducing bacteria using potentiometric stripping analysis
	Wan, Yi 2; Zhang, Dun 1; Hou, Baorong
	2010-09-15
发表期刊	TALANTA
ISSN	0039-9140
卷号	82 期号:4 页码:1608-1611
文章类型	Article
摘要	A fast, sensitive and reliable potentiometric stripping analysis (PSA) is described for the selective detection of the marine pathogenic sulfate-reducing bacterium (SRB). Desulforibrio caledoiensis. The chemical and electrochemical parameters that exert influence on the deposition and stripping of lead ion, such as deposition potential, deposition time and pH value were carefully studied. The concentration of SRB was determined in acetate buffer solution (pH 5.2) under the optimized condition (deposition potential of -1.3 V. deposition time of 250 s, ionic strength of 0.2 mol L(-1) and oxidant mercury (II) concentration of 40 mg L(-1)). A linear relationship between the stripping response and the logarithm of the bacterial concentration was observed in the range of 2.3 x 10 to 2.3 x 10(7) cfu mL(-1). In addition, the potentiometric stripping technique gave a distinct response to the SRB, but had no obvious response to Escherichia coli. The measurement system has a potential for further applications and provides a facile and sample method for detection of pathogenic bacteria. (C) 2010 Elsevier B.V. All rights reserved.; A fast, sensitive and reliable potentiometric stripping analysis (PSA) is described for the selective detection of the marine pathogenic sulfate-reducing bacterium (SRB). Desulforibrio caledoiensis. The chemical and electrochemical parameters that exert influence on the deposition and stripping of lead ion, such as deposition potential, deposition time and pH value were carefully studied. The concentration of SRB was determined in acetate buffer solution (pH 5.2) under the optimized condition (deposition potential of -1.3 V. deposition time of 250 s, ionic strength of 0.2 mol L-1 and oxidant mercury (II) concentration of 40 mg L-1). A linear relationship between the stripping response and the logarithm of the bacterial concentration was observed in the range of 2.3 x 10 to 2.3 x 10(7) cfu mL(-1). In addition, the potentiometric stripping technique gave a distinct response to the SRB, but had no obvious response to Escherichia coli. The measurement system has a potential for further applications and provides a facile and sample method for detection of pathogenic bacteria. (C) 2010 Elsevier B.V. All rights reserved.
关键词	Potentiometric Stripping Analysis Sulfate-reducing Bacteria Pathogenic Bacteria Detection Sulfide
学科领域	Chemistry, Analytical
DOI	10.1016/j.talanta.2010.07.030
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000282480500080
引用统计	被引频次：13[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/5192
专题	海洋环境腐蚀与生物污损重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Corros Sci, Qingdao 266071, Shandong, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Wan, Yi,Zhang, Dun,Hou, Baorong. Selective and specific detection of sulfate-reducing bacteria using potentiometric stripping analysis[J]. TALANTA,2010,82(4):1608-1611.
APA	Wan, Yi,Zhang, Dun,&Hou, Baorong.(2010).Selective and specific detection of sulfate-reducing bacteria using potentiometric stripping analysis.TALANTA,82(4),1608-1611.
MLA	Wan, Yi,et al."Selective and specific detection of sulfate-reducing bacteria using potentiometric stripping analysis".TALANTA 82.4(2010):1608-1611.

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