Molecular characterization and expression of a novel Toll gene from the swimming crab Portunus trituberculatus

doi:10.1016/j.molimm.2015.06.028

	Molecular characterization and expression of a novel Toll gene from the swimming crab Portunus trituberculatus
	Li, Meng1,2 ; Li, Caiwen1 ; Wang, Jinfeng1,2 ; Song, Shuqun 1
	2015-10-01
发表期刊	MOLECULAR IMMUNOLOGY
卷号	67 期号:2 页码:388-397
文章类型	Article
摘要	Tolls/Toll-like receptors (TLRs) are important cell-surface receptors serving as pattern recognition receptors (PRRs) in the Tolls/TLRs signaling pathway of innate immune responses. In the present study, we isolated and characterized a novel Toll gene (PtToll) from Portunus trituberculatus, and further investigated its expression in various tissues of crab hosts challenged with the parasitic dinoflagellate Hematodinium. The full-length cDNA of PtToll was 3745 bp, with a 3012 bp open reading frame (ORF) encoding 1003 amino acids. Conserved domains consist of 15 tandem leucine-rich repeats (LRRs), a single-pass transmembrane segment (TM) and a cytoplasmic Toll/interleukin-1R (TIR) domain. The PtToll protein shared high similarity to other crustacean Tolls and was clustered with the crustacean Tolls in the phylogenetic tree. The PtToll gene was constitutively expressed in various tissues of P. trituberculatus, with the highest expression in hemocytes. After being challenged with the parasite, the transcripts of PtToll reacted immediately with significant alterations in all the tested tissues, and decreased consistently in most of the detected tissues (e.g., hemocytes, gill, heart, and muscle) within 24 h. Then the transcripts of PtToll were significantly up-regulated in hemocytes and heart at 48 h, and in hepatopancreas at 48 and 96 h post the parasitic challenge. By 192 h post challenge, the transcriptional level of PtToll indicated a significant suppression or a decreasing trend. The fluctuations of PtToll gene expression suggested that PtToll was closely associated with intrusion of the Hematodiniurn parasites, and may possess a vital and systematic function in the innate immunity of P. trituberculatus against the parasitic infection. (C) 2015 Elsevier Ltd. All rights reserved.
关键词	Crustacean Toll Receptor Transcript Expression Innate Immunity Parasite
DOI	10.1016/j.molimm.2015.06.028
收录类别	SCI
语种	英语
WOS记录号	WOS:000361922400023
引用统计	被引频次：18[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/50937
专题	海洋生态与环境科学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Li, Meng,Li, Caiwen,Wang, Jinfeng,et al. Molecular characterization and expression of a novel Toll gene from the swimming crab Portunus trituberculatus[J]. MOLECULAR IMMUNOLOGY,2015,67(2):388-397.
APA	Li, Meng,Li, Caiwen,Wang, Jinfeng,&Song, Shuqun.(2015).Molecular characterization and expression of a novel Toll gene from the swimming crab Portunus trituberculatus.MOLECULAR IMMUNOLOGY,67(2),388-397.
MLA	Li, Meng,et al."Molecular characterization and expression of a novel Toll gene from the swimming crab Portunus trituberculatus".MOLECULAR IMMUNOLOGY 67.2(2015):388-397.

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