Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Characterization of a novel alkaline arylsulfatase from Marinomonas sp FW-1 and its application in the desulfation of red seaweed agar | |
Wang, Xueyan1; Duan, Delin2; Xu, Jiachao1 ; Gao, Xin1; Fu, Xiaoting1
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| 2015-10-01 | |
| 发表期刊 | JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
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| 卷号 | 42期号:10页码:1353-1362 |
| 文章类型 | Article |
| 摘要 | A bacterial strain capable of hydrolyzing sulfate ester bonds of p-nitrophenyl sulfate (pNPS) and agar was isolated from the coast area of Qingdao, China. It was identified as Marinomonas based on its 16S rRNA gene sequence and named as Marinomonas sp. FW-1. An arylsulfatase with a recovery of 13 % and a fold of 12 was purified to a homogeneity using ion exchange and gel filtration chromatographies. The enzyme was composed of a single polypeptide chain with the molecular mass of 33 kDa estimated using SDS-PAGE. The optimal pH and temperature of arylsulfatase were pH 9.0 and 45, respectively. Arylsulfatase was stable over pH 8-11 and at temperature below 55 A degrees C. The K (m) and V (max) of this enzyme for the hydrolysis of pNPS were determined to be 13.73 and 270.27 mu M/min, respectively. The desulfation ratio against agar from red seaweed Gelidium amansii and Gracilaria lemaneiformis were 86.11 and 89.61 %, respectively. There was no difference between the DNA electrophoresis spectrum on the gel of the arylsulfatase-treated G. amansii agar and that of the commercial agarose. Therefore, this novel alkaline arylsulfatase might have a great potential for application in enzymatic conversion of agar to agarose. |
| 关键词 | Arylsulfatase Desulfation Agarose Purification Characterization |
| DOI | 10.1007/s10295-015-1625-6 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000361650000006 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/50884 |
| 专题 | 实验海洋生物学重点实验室 |
| 作者单位 | 1.Ocean Univ China, Coll Food Sci & Engn, Qingdao 266003, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Xueyan,Duan, Delin,Xu, Jiachao,et al. Characterization of a novel alkaline arylsulfatase from Marinomonas sp FW-1 and its application in the desulfation of red seaweed agar[J]. JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY,2015,42(10):1353-1362. |
| APA | Wang, Xueyan,Duan, Delin,Xu, Jiachao,Gao, Xin,&Fu, Xiaoting.(2015).Characterization of a novel alkaline arylsulfatase from Marinomonas sp FW-1 and its application in the desulfation of red seaweed agar.JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY,42(10),1353-1362. |
| MLA | Wang, Xueyan,et al."Characterization of a novel alkaline arylsulfatase from Marinomonas sp FW-1 and its application in the desulfation of red seaweed agar".JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY 42.10(2015):1353-1362. |
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