The broad pattern recognition spectrum of the Toll-like receptor in mollusk Zhikong scallop Chlamys farreri

doi:10.1016/j.dci.2015.05.011

IOCAS-IR > 实验海洋生物学重点实验室

	The broad pattern recognition spectrum of the Toll-like receptor in mollusk Zhikong scallop Chlamys farreri
	Wang, Mengqiang1 ; Wang, Lingling 1; Guo, Ying 1; Sun, Rui 1; Yue, Feng 1; Yi, Qilin 1; Song, Linsheng 1,2
	2015-10-01
发表期刊	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
卷号	52 期号:2 页码:192-201
文章类型	Article
摘要	Toll-like receptors (TLRs) are among the most studied pattern recognition receptors (PRRs) playing essential roles in innate immune defenses. In the present study, the basic features of CfTLR in mollusk Zhikong scallop Chlamys jarred. including sequence homology, tissue distribution, subcellular localization and ligands spectrum, were investigated to elucidate its pattern recognition. The elements of extracellular domains (ECD) in CfTLR displayed high homology to the corresponding parts of the ECDs in TLRs from Homo sapiens. CfTLR protein was detected in hemocytes, mantle, gills, hepatopancreas, kidney and gonad of the scallops, and it was localized in both the plasma membranes and the lysosomes in HEK293T cells. MLR could activate NF kappa B in response to multiple HsTLR ligands including Pam3CSK4, glucan (GLU), peptidoglycan (PGN), polyriboinosinic:polyribocytidylic acid (poly I:C), Imiquimod and three types of CpG. Additionally, the scallop serum could enhance the induction of NF kappa B in the CfTLR expressing cells elicited by most PAMPs, including GLU, PGN, Imiquimod and four types of CpG. It could be concluded that this primitive mollusk TLR shared a hybrid function in pattern recognition and could recognize broader ligands than mammalian TLRs, and its mosaic capability of pathogen associated molecular pattern (PAMP) recognition might be based on the basic features of its structure, ligand properties and the assistance of some components in scallop serum. (C) 2015 Elsevier Ltd. All rights reserved.
关键词	Chlamys Farreri Innate Immunity Pattern Recognition Toll-like Receptor
DOI	10.1016/j.dci.2015.05.011
收录类别	SCI
语种	英语
WOS记录号	WOS:000360592200010
引用统计	被引频次：52[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/50762
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China 2.Dalian Ocean Univ, Dalian 116023, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Wang, Mengqiang,Wang, Lingling,Guo, Ying,et al. The broad pattern recognition spectrum of the Toll-like receptor in mollusk Zhikong scallop Chlamys farreri[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2015,52(2):192-201.
APA	Wang, Mengqiang.,Wang, Lingling.,Guo, Ying.,Sun, Rui.,Yue, Feng.,...&Song, Linsheng.(2015).The broad pattern recognition spectrum of the Toll-like receptor in mollusk Zhikong scallop Chlamys farreri.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,52(2),192-201.
MLA	Wang, Mengqiang,et al."The broad pattern recognition spectrum of the Toll-like receptor in mollusk Zhikong scallop Chlamys farreri".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 52.2(2015):192-201.

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