Recombinant expression and functional analysis of an isoform of anti-lipopolysaccharide factors (FcALF5) from Chinese shrimp Fenneropenaeus chinensis

doi:10.1016/j.dci.2015.06.015

IOCAS-IR > 实验海洋生物学重点实验室

	Recombinant expression and functional analysis of an isoform of anti-lipopolysaccharide factors (FcALF5) from Chinese shrimp Fenneropenaeus chinensis
	Yang, Hui1,2 ; Li, Shihao 1,3; Li, Fuhua 1,3; Lv, Xinjia1,2 ; Xiang, Jianhai1
	2015-11-01
发表期刊	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
卷号	53 期号:1 页码:47-54
文章类型	Article
摘要	Antimicrobial peptides (AMPs) have a great potential to be used as a substitute for antibiotics since AMPs don't lead to bacteria's drug resistance. Anti-lipopolysaccharide factors (ALFs) are one type of AMPs and exist in crustaceans. In the present study, we produced a recombinant protein (rFcALF5) of an ALP isoform (FcALF5) from Chinese shrimp Fenneropenaeus chinensis through a prokaryotic expression system. The rFcALF5 exhibited varied antibacterial activities against different bacteria. Besides its antibacterial activities, it could also inhibit the infection of white spot syndrome virus (WSSV) to shrimp after preincubation with this virus. In order to learn the antiviral mechanism on how rFcALF5 influences WSSV infection, the interaction between the total proteins of WSSV and rFcALF5 was analyzed and the data showed that rFcALF5 had direct interaction with the envelope protein VP24 of WSSV. The LPS binding domain (LBD) of FcALF5 also showed direct interaction with VP24 of WSSV. Therefore we inferred that the antiviral activity of FcALF5 might be achieved through the binding of its LBD to VP24 of WSSV. These findings provided more information to develop new strategies for the control of shrimp disease in aquaculture. (C) 2015 Elsevier Ltd. All rights reserved.
关键词	Anti-lipopolysaccharide Factors Antibacterial Antiviral Vp24 Wssv
DOI	10.1016/j.dci.2015.06.015
收录类别	SCI
语种	英语
WOS记录号	WOS:000360772900006
引用统计	被引频次：37[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/50421
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Yang, Hui,Li, Shihao,Li, Fuhua,et al. Recombinant expression and functional analysis of an isoform of anti-lipopolysaccharide factors (FcALF5) from Chinese shrimp Fenneropenaeus chinensis[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2015,53(1):47-54.
APA	Yang, Hui,Li, Shihao,Li, Fuhua,Lv, Xinjia,&Xiang, Jianhai.(2015).Recombinant expression and functional analysis of an isoform of anti-lipopolysaccharide factors (FcALF5) from Chinese shrimp Fenneropenaeus chinensis.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,53(1),47-54.
MLA	Yang, Hui,et al."Recombinant expression and functional analysis of an isoform of anti-lipopolysaccharide factors (FcALF5) from Chinese shrimp Fenneropenaeus chinensis".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 53.1(2015):47-54.

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