A new pattern of primordial germ cell migration in olive flounder (Paralichthys olivaceus) identified using nanos3

doi:10.1007/s00427-015-0503-6

IOCAS-IR > 实验海洋生物学重点实验室

	A new pattern of primordial germ cell migration in olive flounder (Paralichthys olivaceus) identified using nanos3
	Li, Meijie1,2 ; Tan, Xungang1 ; Jiao, Shuang 1; Wang, Qian1 ; Wu, Zhihao 1; You, Feng 1; Zou, Yuxia 1
	2015-07-01
发表期刊	DEVELOPMENT GENES AND EVOLUTION
卷号	225 期号:4 页码:195-206
文章类型	Article
摘要	The olive flounder (Paralichthys olivaceus) is an important cultured marine fish. However, little information is available on primordial germ cell (PGC) development and migration in this species; such information is vital for applications in artificial reproduction and for the preservation of genetic resources. Here, we sought to remedy this information deficit by isolating the germline-specific gene nanos3 and analyzing its expression in olive flounder. Sequencing analysis showed that olive flounder nanos3 contained a typical RNA-binding zinc finger domain. A phylogenetic analysis demonstrated that nanos3 of the olive flounder grouped with that of the barfin flounder (Verasper moseri). In the olive flounder, nanos3 was consistently expressed during embryogenesis. Whole-mount in situ hybridization showed that a new pattern of PGC migration was present in olive flounder, which combined elements of the PGC migration patterns of medaka, herring, and goby. In olive flounder, PGCs aligned along the lateral plate mesoderm in two loose, elongated lines at early embryogenesis, aggregated into a single loose cluster at mid-embryogenesis, then re-aligned into two tight clusters at late somitogenesis, and finally migrated to the genital ridge as two clusters. Furthermore, whole-mount in situ hybridization revealed that expression of stromal derived factor 1 (Sdf1) was important for guiding of PGC migration during somitogenesis. In particular, Sdf1 directed aggregation of PGCs into a single loose cluster from the two elongated lines during mid-embryogenesis. Additionally, PGCs in zebrafish were successfully visualized by injection of chimeric RNA containing the green fluorescent protein (GFP) and 3' untranslated region of olive flounder nanos3. These findings provide new insights into PGC migration and development in olive flounder and will also facilitate germ cell manipulation in this species.
关键词	Teleost Fish Pgc Migration Nanos3 Sdf1 Expression Pattern
DOI	10.1007/s00427-015-0503-6
收录类别	SCI
语种	英语
WOS记录号	WOS:000358876800001
引用统计	被引频次：22[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/49613
专题	实验海洋生物学重点实验室海洋地质与环境重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Li, Meijie,Tan, Xungang,Jiao, Shuang,et al. A new pattern of primordial germ cell migration in olive flounder (Paralichthys olivaceus) identified using nanos3[J]. DEVELOPMENT GENES AND EVOLUTION,2015,225(4):195-206.
APA	Li, Meijie.,Tan, Xungang.,Jiao, Shuang.,Wang, Qian.,Wu, Zhihao.,...&Zou, Yuxia.(2015).A new pattern of primordial germ cell migration in olive flounder (Paralichthys olivaceus) identified using nanos3.DEVELOPMENT GENES AND EVOLUTION,225(4),195-206.
MLA	Li, Meijie,et al."A new pattern of primordial germ cell migration in olive flounder (Paralichthys olivaceus) identified using nanos3".DEVELOPMENT GENES AND EVOLUTION 225.4(2015):195-206.

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