Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Pseudomonas fluorescens: identification of Fur-regulated proteins and evaluation of their contribution to pathogenesis | |
Liu, Li1,2 ; Chi, Heng1; Sun, Li1
| |
| 2015-06-29 | |
| 发表期刊 | DISEASES OF AQUATIC ORGANISMS
 |
| 卷号 | 115期号:1页码:67-80 |
| 文章类型 | Article |
| 摘要 | Pseudomonas fluorescens is a Gram-negative bacterium and a common pathogen to a wide range of farmed fish. In a previous study, we found that the ferric uptake regulator gene (fur) is essential to the infectivity of a pathogenic fish isolate of P. fluorescens (wild-type strain TSS). In the present work, we conducted comparative proteomic analysis to examine the global protein profiles of TSS and the P. fluorescens fur knockout mutant TFM. Twenty-eight differentially produced proteins were identified, which belong to different functional categories. Four of these proteins, viz. TssP (a type VI secretion protein), PspA (a serine protease), OprF (an outer membrane porin), and ClpP (the proteolytic subunit of an ATP-dependent Clp protease), were assessed for virulence participation in a model of turbot Scophthalmus maximus. The results showed that the oprF and clpP knockouts exhibited significantly reduced capacities in (1) resistance against the bactericidal effect of host serum, (2) dissemination into and colonization of host tissues, and (3) inducing host mortality. In contrast, mutation of tssP and pspA had no apparent effect on the pathogenicity of TSS. Purified recombinant OprF, when used as a subunit vaccine, induced production of specific serum antibodies in immunized fish and elicited significant protection against lethal TSS challenge. Antibody blocking of the OprF in TSS significantly impaired the ability of the bacteria to invade host tissues. Taken together, these results indicate for the first time that in pathogenic P. fluorescens, Fur regulates the expression of diverse proteins, some of which are required for optimal infection. |
| 关键词 | Pseudomonas Fluorescens Ferric Uptake Regulator Proteomic Infection Virulence Vaccine |
| DOI | 10.3354/dao02874 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000357598200008 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/49204 |
| 专题 | 实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Liu, Li,Chi, Heng,Sun, Li. Pseudomonas fluorescens: identification of Fur-regulated proteins and evaluation of their contribution to pathogenesis[J]. DISEASES OF AQUATIC ORGANISMS,2015,115(1):67-80. |
| APA | Liu, Li,Chi, Heng,&Sun, Li.(2015).Pseudomonas fluorescens: identification of Fur-regulated proteins and evaluation of their contribution to pathogenesis.DISEASES OF AQUATIC ORGANISMS,115(1),67-80. |
| MLA | Liu, Li,et al."Pseudomonas fluorescens: identification of Fur-regulated proteins and evaluation of their contribution to pathogenesis".DISEASES OF AQUATIC ORGANISMS 115.1(2015):67-80. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Pseudomonas fluoresc（781KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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