Diversity of Both the Cultivable Protease-Producing Bacteria and Their Extracellular Proteases in the Sediments of the South China Sea

doi:10.1007/s00248-009-9506-z

IOCAS-IR > 海洋地质与环境重点实验室

	Diversity of Both the Cultivable Protease-Producing Bacteria and Their Extracellular Proteases in the Sediments of the South China Sea
	Zhou, Ming-Yang 1; Chen, Xiu-Lan 1; Zhao, Hui-Lin 1; Dang, Hong-Yue 2; Luan, Xi-Wu 3; Zhang, Xi-Ying 1; He, Hai-Lun 1; Zhou, Bai-Cheng 1; Zhang, Yu-Zhong 1
	2009-10-01
发表期刊	MICROBIAL ECOLOGY
ISSN	0095-3628
卷号	58 期号:3 页码:582-590
文章类型	Article
摘要	Protease-producing bacteria are known to play an important role in degrading sedimentary particular organic nitrogen, and yet, their diversity and extracellular proteases remain largely unknown. In this paper, the diversity of the cultivable protease-producing bacteria and their extracellular proteases in the sediments of the South China Sea was investigated. The richness of the cultivable protease-producing bacteria reached 10(6) cells/g in all sediment samples. Analysis of the 16S rRNA gene sequences revealed that the predominant cultivated protease-producing bacteria are Gammaproteobacteria affiliated with the genera Pseudoalteromonas, Alteromonas, Marinobacter, Idiomarina, Halomonas, Vibrio, Shewanella, Pseudomonas, and Rheinheimera, with Alteromonas (34.6%) and Pseudoalteromonas (28.2%) as the predominant groups. Inhibitor analysis showed that nearly all the extracellular proteases from the bacteria are serine proteases or metalloproteases. Moreover, these proteases have different hydrolytic ability to different proteins, reflecting they may belong to different kinds of serine proteases or metalloproteases. To our knowledge, this study represents the first report of the diversity of bacterial proteases in deep-sea sediments.; Protease-producing bacteria are known to play an important role in degrading sedimentary particular organic nitrogen, and yet, their diversity and extracellular proteases remain largely unknown. In this paper, the diversity of the cultivable protease-producing bacteria and their extracellular proteases in the sediments of the South China Sea was investigated. The richness of the cultivable protease-producing bacteria reached 10(6) cells/g in all sediment samples. Analysis of the 16S rRNA gene sequences revealed that the predominant cultivated protease-producing bacteria are Gammaproteobacteria affiliated with the genera Pseudoalteromonas, Alteromonas, Marinobacter, Idiomarina, Halomonas, Vibrio, Shewanella, Pseudomonas, and Rheinheimera, with Alteromonas (34.6%) and Pseudoalteromonas (28.2%) as the predominant groups. Inhibitor analysis showed that nearly all the extracellular proteases from the bacteria are serine proteases or metalloproteases. Moreover, these proteases have different hydrolytic ability to different proteins, reflecting they may belong to different kinds of serine proteases or metalloproteases. To our knowledge, this study represents the first report of the diversity of bacterial proteases in deep-sea sediments.
关键词	Deep-sea Subseafloor Sediments Seep Carbonates Sp Sm9913 Psychrotolerant Communities Strain Ocean
学科领域	Ecology ; Marine & Freshwater Biology ; Microbiology
DOI	10.1007/s00248-009-9506-z
收录类别	SCI
语种	英语
WOS记录号	WOS:000269928300014
引用统计	被引频次：101[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/3734
专题	海洋地质与环境重点实验室
作者单位	1.Shandong Univ, State Key Lab Microbial Technol, Marine Biotechnol Res Ctr, Jinan 250100, Peoples R China 2.China Univ Petr E China, Ctr Bioengn & Biotechnol, Qingdao 266555, Peoples R China 3.Chinese Acad Sci, Key Lab Marine Geol & Environm, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Zhou, Ming-Yang,Chen, Xiu-Lan,Zhao, Hui-Lin,et al. Diversity of Both the Cultivable Protease-Producing Bacteria and Their Extracellular Proteases in the Sediments of the South China Sea[J]. MICROBIAL ECOLOGY,2009,58(3):582-590.
APA	Zhou, Ming-Yang.,Chen, Xiu-Lan.,Zhao, Hui-Lin.,Dang, Hong-Yue.,Luan, Xi-Wu.,...&Zhang, Yu-Zhong.(2009).Diversity of Both the Cultivable Protease-Producing Bacteria and Their Extracellular Proteases in the Sediments of the South China Sea.MICROBIAL ECOLOGY,58(3),582-590.
MLA	Zhou, Ming-Yang,et al."Diversity of Both the Cultivable Protease-Producing Bacteria and Their Extracellular Proteases in the Sediments of the South China Sea".MICROBIAL ECOLOGY 58.3(2009):582-590.

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