| Extracellular hydrolytic enzyme screening of culturable heterotrophic bacteria from deep-sea sediments of the Southern Okinawa Trough | |
Dang, Hongyue1,2; Zhu, Hu2; Wang, Jing2; Li, Tiegang1
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| 2009 | |
| 发表期刊 | WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
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| ISSN | 0959-3993 |
| 卷号 | 25期号:1页码:71-79 |
| 文章类型 | Article |
| 摘要 | The Southern Okinawa Trough is an area of focused sedimentation due to particulate matter export from the shelf of the East China Sea and the island of Taiwan. In order to understand the geomicrobiological characteristics of this unique sedimentary environment, bacterial cultivations were carried out for an 8.61 m CASQ core sediment sample. A total of 98 heterotrophic bacterial isolates were characterized based on 16S rRNA gene phylogenetic analysis. These isolates can be grouped into four bacterial divisions, including 13 genera and more than 20 species. Bacteria of the gamma-Proteobacteria lineage, especially those from the Halomonas ( 27 isolates) and Psychrobacter ( 20 isolates) groups, dominate in the culturable bacteria assemblage. They also have the broadest distribution along the depth of the sediment. More than 72.4% of the isolates showed extracellular hydrolytic enzyme activities, such as amylases, proteases, lipases and Dnases, and nearly 59.2% were cold-adapted exoenzyme-producers. Several Halomonas strains show almost all the tested hydrolases activities. The wide distribution of exoenzyme activities in the isolates may indicate their important ecological role of element biogeochemical cycling in the studied deep-sea sedimentary environment.; The Southern Okinawa Trough is an area of focused sedimentation due to particulate matter export from the shelf of the East China Sea and the island of Taiwan. In order to understand the geomicrobiological characteristics of this unique sedimentary environment, bacterial cultivations were carried out for an 8.61 m CASQ core sediment sample. A total of 98 heterotrophic bacterial isolates were characterized based on 16S rRNA gene phylogenetic analysis. These isolates can be grouped into four bacterial divisions, including 13 genera and more than 20 species. Bacteria of the gamma-Proteobacteria lineage, especially those from the Halomonas ( 27 isolates) and Psychrobacter ( 20 isolates) groups, dominate in the culturable bacteria assemblage. They also have the broadest distribution along the depth of the sediment. More than 72.4% of the isolates showed extracellular hydrolytic enzyme activities, such as amylases, proteases, lipases and Dnases, and nearly 59.2% were cold-adapted exoenzyme-producers. Several Halomonas strains show almost all the tested hydrolases activities. The wide distribution of exoenzyme activities in the isolates may indicate their important ecological role of element biogeochemical cycling in the studied deep-sea sedimentary environment. |
| 关键词 | Heterotrophic Bacteria Extracellular Hydrolytic Enzyme Exoenzyme Cold-adaptivity Extremophile Deep-sea Sediment Southern Okinawa Trough West Pacific Ocean |
| 学科领域 | Biotechnology & Applied Microbiology |
| DOI | 10.1007/s11274-008-9865-5 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000261413100010 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/3658 |
| 专题 | 海洋地质与环境重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Geol & Environm, Qingdao 266071, Peoples R China 2.China Univ Petr E China, Ctr Bioengn & Biotechnol, Qingdao 266555, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Dang, Hongyue,Zhu, Hu,Wang, Jing,et al. Extracellular hydrolytic enzyme screening of culturable heterotrophic bacteria from deep-sea sediments of the Southern Okinawa Trough[J]. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,2009,25(1):71-79. |
| APA | Dang, Hongyue,Zhu, Hu,Wang, Jing,&Li, Tiegang.(2009).Extracellular hydrolytic enzyme screening of culturable heterotrophic bacteria from deep-sea sediments of the Southern Okinawa Trough.WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,25(1),71-79. |
| MLA | Dang, Hongyue,et al."Extracellular hydrolytic enzyme screening of culturable heterotrophic bacteria from deep-sea sediments of the Southern Okinawa Trough".WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 25.1(2009):71-79. |
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