Antioxidant activity of differently regioselective chitosan sulfates in vitro

doi:10.1016/j.bmc.2004.11.002

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	Antioxidant activity of differently regioselective chitosan sulfates in vitro
	Xing, RE; Yu, HH; Liu, S ; Zhang, WW ; Zhang, QB ; Li, Z; Li, PC
	2005-02-15
发表期刊	BIOORGANIC & MEDICINAL CHEMISTRY
ISSN	0968-0896
卷号	13 期号:4 页码:1387-1392
文章类型	Article
摘要	Differently regioselective chitosan sulfates were prepared according to Hanno Baumann's methods. Their antioxidant potencies were investigated employing various established in vitro systems, such as 1,1-diphenyl-2-picrylhydrazyl (DPPH)/superoxide/hydroxyl radicals scavenging, reducing power, iron ion chelating and total antioxidant activity. All kinds of sulfated chitosans (HCTS, TSCTS, SCTS, TCTS) showed strong inhibitory activity toward superoxide radical by the PMS-NADH system compared to Vc. According to the above-mentioned order their IC(50) were 0.012, 0.040, 0.015, 0.022mg/mL, respectively, however, scavenging activity of Vc on superoxide radical was 68.19% at 2.0mg/mL. Scavenging activity of superoxide radical was found to be in the order of HCTS > SCTS > TCTS > TSCTS > Vc. Furthermore, all kinds of sulfated chitosans exhibited strong concentration-dependent inhibition of deoxyribose oxidation. Except for HCTS, others had stronger scavenging activity on hydroxyl radical than Vc. Scavenging effect of TSCTS on 1, 1 -diphenyl-2-picrylhydrazy] radical was little lower than that of BHA, but better than that of others. All kinds of sulfated chitosans were efficient in the reducing power, especially TSCTS. TSCTS and TCTS showed considerable ferrous ion chelating potency. The data obtained in vitro models clearly establish the antioxidant potency of all kinds of sulfated chitosans. These in vitro results suggested the possibility that sulfated chitosans could be effectively employed as ingredient in health or functional food, to alleviate oxidative stress. However, comprehensive studies need to be conducted to ascertain the in vivo safety of sulfated chitosans in experimental animal models. (C) 2004 Elsevier Ltd. All rights reserved.; Differently regioselective chitosan sulfates were prepared according to Hanno Baumann's methods. Their antioxidant potencies were investigated employing various established in vitro systems, such as 1,1-diphenyl-2-picrylhydrazyl (DPPH)/superoxide/hydroxyl radicals scavenging, reducing power, iron ion chelating and total antioxidant activity. All kinds of sulfated chitosans (HCTS, TSCTS, SCTS, TCTS) showed strong inhibitory activity toward superoxide radical by the PMS-NADH system compared to Vc. According to the above-mentioned order their IC50 were 0.012, 0.040, 0.015, 0.022mg/mL, respectively, however, scavenging activity of Vc on superoxide radical was 68.19% at 2.0mg/mL. Scavenging activity of superoxide radical was found to be in the order of HCTS > SCTS > TCTS > TSCTS > Vc. Furthermore, all kinds of sulfated chitosans exhibited strong concentration-dependent inhibition of deoxyribose oxidation. Except for HCTS, others had stronger scavenging activity on hydroxyl radical than Vc. Scavenging effect of TSCTS on 1, 1 -diphenyl-2-picrylhydrazy] radical was little lower than that of BHA, but better than that of others. All kinds of sulfated chitosans were efficient in the reducing power, especially TSCTS. TSCTS and TCTS showed considerable ferrous ion chelating potency. The data obtained in vitro models clearly establish the antioxidant potency of all kinds of sulfated chitosans. These in vitro results suggested the possibility that sulfated chitosans could be effectively employed as ingredient in health or functional food, to alleviate oxidative stress. However, comprehensive studies need to be conducted to ascertain the in vivo safety of sulfated chitosans in experimental animal models. (C) 2004 Elsevier Ltd. All rights reserved.
关键词	All Kinds Of Sulfated Chitosans Antioxidant Activity Reducing Power Chelating Effect Radical Scavenging Effect
学科领域	Biochemistry & Molecular Biology ; Chemistry, Medicinal ; Chemistry, Organic
DOI	10.1016/j.bmc.2004.11.002
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000226967900045
引用统计	被引频次：209[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/3540
专题	海洋生物技术研发中心海洋环境工程技术研究发展中心
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Xing, RE,Yu, HH,Liu, S,et al. Antioxidant activity of differently regioselective chitosan sulfates in vitro[J]. BIOORGANIC & MEDICINAL CHEMISTRY,2005,13(4):1387-1392.
APA	Xing, RE.,Yu, HH.,Liu, S.,Zhang, WW.,Zhang, QB.,...&Li, PC.(2005).Antioxidant activity of differently regioselective chitosan sulfates in vitro.BIOORGANIC & MEDICINAL CHEMISTRY,13(4),1387-1392.
MLA	Xing, RE,et al."Antioxidant activity of differently regioselective chitosan sulfates in vitro".BIOORGANIC & MEDICINAL CHEMISTRY 13.4(2005):1387-1392.

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