Topographic changes in nascent and early mesoderm in amphioxus embryos studied by Dil labeling and by in situ hybridization for a Brachyury gene

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	Topographic changes in nascent and early mesoderm in amphioxus embryos studied by Dil labeling and by in situ hybridization for a Brachyury gene
	Zhang, SC; Holland, ND; Holland, LZ
	1997
发表期刊	DEVELOPMENT GENES AND EVOLUTION
ISSN	0949-944X
卷号	206 期号:8 页码:532-535
摘要	In amphioxus embryos, the nascent and early mesoderm (including chorda-mesoderm) was visualized by expression of a Brachyury gene (AmBra-2). A band of mesoderm is first detected encircling the earliest (vegetal plate stage) gastrula sub-equatorially. Soon thereafter, the vegetal plate invaginates. resulting in a cap-shaped gastrula with the mesoderm localized at the blastoporal lip and completely encircling the blastopore. As the gastrula stage progresses, DiI (a vital dye) labeling demonstrates that the entire mesoderm is internalized by a slight involution of the epiblast into the hypoblast all around the perimeter of the blastopore. Subsequently. during the early neurula stage, the internalized mesoderm undergoes anterior extension mid-dorsally (as notochord) and dorsolaterally (in paraxial regions when segments will later form). By the late neurula stage, AmBra-2 is no longer transcribed throughout the mesoderm as a whole; instead. expression is detectable only in the posterior mesoderm and in the notochord, but not in par axial mesoderm where definitive somites have formed.
关键词	Branchiostoma-belcheri
学科领域	Cell Biology ; Evolutionary Biology ; Developmental Biology
收录类别	sci
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/3388
专题	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Zhang, SC,Holland, ND,Holland, LZ. Topographic changes in nascent and early mesoderm in amphioxus embryos studied by Dil labeling and by in situ hybridization for a Brachyury gene[J]. DEVELOPMENT GENES AND EVOLUTION,1997,206(8):532-535.
APA	Zhang, SC,Holland, ND,&Holland, LZ.(1997).Topographic changes in nascent and early mesoderm in amphioxus embryos studied by Dil labeling and by in situ hybridization for a Brachyury gene.DEVELOPMENT GENES AND EVOLUTION,206(8),532-535.
MLA	Zhang, SC,et al."Topographic changes in nascent and early mesoderm in amphioxus embryos studied by Dil labeling and by in situ hybridization for a Brachyury gene".DEVELOPMENT GENES AND EVOLUTION 206.8(1997):532-535.

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