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Spectroscopic properties of the C-phycocyanin-allophycocyanin conjugate and the isolated phycobilisomes from Spirulina platensis
Wang, GC; Zhou, BC; Tseng, CK
1997
发表期刊PHOTOSYNTHETICA
ISSN0300-3604
卷号34期号:1页码:57-65
通讯作者Wang, GC()
摘要C-phycocyanin (CPC) and allophycocyanin (APC) were purified from Spirulina platensis, then the CPC was attached covalently to the APC by reacting their epsilon-amino groups. The excitation energy could be transferred from the CPC to the APC in the CPC-APC conjugate. Intact phycobilisomes (PBS), consisting of CPC, APC, colourless linker polypeptides, and APC B or L-cm, were isolated from S. platensis. Spectroscopic properties of the isolated PBSs kept at 20 degrees C for various times showed that the connection between the APC and the APC B or L-cm was looser than that between the CPC and the APC in the isolated PBSs. The CPC-APC conjugate was more stable than the isolated PBSs, and the linker polypeptides had a minor influence on the excitation energy transfer characteristic between different phycobiliproteins in the PBS.
关键词absorption and fluorescence emission and excitation spectra covalent bond linker polypeptides
收录类别SCI
语种英语
WOS研究方向Plant Sciences
WOS类目Plant Sciences
WOS记录号WOS:A1997YE41400007
出版者INST EXPERIMENTAL BOTANY, ACAD SCI CZECH REPUBLIC
引用统计
被引频次:10[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/3382
专题实验海洋生物学重点实验室
通讯作者Wang, GC
作者单位CHINESE ACAD SCI,INST OCEANOL,EXPT MARINE BIOL LAB,QINGDAO 266071,PEOPLES R CHINA
第一作者单位中国科学院海洋研究所
通讯作者单位中国科学院海洋研究所
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GB/T 7714
Wang, GC,Zhou, BC,Tseng, CK. Spectroscopic properties of the C-phycocyanin-allophycocyanin conjugate and the isolated phycobilisomes from Spirulina platensis[J]. PHOTOSYNTHETICA,1997,34(1):57-65.
APA Wang, GC,Zhou, BC,&Tseng, CK.(1997).Spectroscopic properties of the C-phycocyanin-allophycocyanin conjugate and the isolated phycobilisomes from Spirulina platensis.PHOTOSYNTHETICA,34(1),57-65.
MLA Wang, GC,et al."Spectroscopic properties of the C-phycocyanin-allophycocyanin conjugate and the isolated phycobilisomes from Spirulina platensis".PHOTOSYNTHETICA 34.1(1997):57-65.
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