Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Spectroscopic properties of the C-phycocyanin-allophycocyanin conjugate and the isolated phycobilisomes from Spirulina platensis | |
Wang, GC; Zhou, BC; Tseng, CK | |
1997 | |
发表期刊 | PHOTOSYNTHETICA |
ISSN | 0300-3604 |
卷号 | 34期号:1页码:57-65 |
通讯作者 | Wang, GC() |
摘要 | C-phycocyanin (CPC) and allophycocyanin (APC) were purified from Spirulina platensis, then the CPC was attached covalently to the APC by reacting their epsilon-amino groups. The excitation energy could be transferred from the CPC to the APC in the CPC-APC conjugate. Intact phycobilisomes (PBS), consisting of CPC, APC, colourless linker polypeptides, and APC B or L-cm, were isolated from S. platensis. Spectroscopic properties of the isolated PBSs kept at 20 degrees C for various times showed that the connection between the APC and the APC B or L-cm was looser than that between the CPC and the APC in the isolated PBSs. The CPC-APC conjugate was more stable than the isolated PBSs, and the linker polypeptides had a minor influence on the excitation energy transfer characteristic between different phycobiliproteins in the PBS. |
关键词 | absorption and fluorescence emission and excitation spectra covalent bond linker polypeptides |
收录类别 | SCI |
语种 | 英语 |
WOS研究方向 | Plant Sciences |
WOS类目 | Plant Sciences |
WOS记录号 | WOS:A1997YE41400007 |
出版者 | INST EXPERIMENTAL BOTANY, ACAD SCI CZECH REPUBLIC |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.qdio.ac.cn/handle/337002/3382 |
专题 | 实验海洋生物学重点实验室 |
通讯作者 | Wang, GC |
作者单位 | CHINESE ACAD SCI,INST OCEANOL,EXPT MARINE BIOL LAB,QINGDAO 266071,PEOPLES R CHINA |
第一作者单位 | 中国科学院海洋研究所 |
通讯作者单位 | 中国科学院海洋研究所 |
推荐引用方式 GB/T 7714 | Wang, GC,Zhou, BC,Tseng, CK. Spectroscopic properties of the C-phycocyanin-allophycocyanin conjugate and the isolated phycobilisomes from Spirulina platensis[J]. PHOTOSYNTHETICA,1997,34(1):57-65. |
APA | Wang, GC,Zhou, BC,&Tseng, CK.(1997).Spectroscopic properties of the C-phycocyanin-allophycocyanin conjugate and the isolated phycobilisomes from Spirulina platensis.PHOTOSYNTHETICA,34(1),57-65. |
MLA | Wang, GC,et al."Spectroscopic properties of the C-phycocyanin-allophycocyanin conjugate and the isolated phycobilisomes from Spirulina platensis".PHOTOSYNTHETICA 34.1(1997):57-65. |
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