Isolation, properties and spatial site analysis of gamma subunits of B-phycoerythrin and R-phycoerythrin

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	Isolation, properties and spatial site analysis of gamma subunits of B-phycoerythrin and R-phycoerythrin
	Wang, GG; Zhou, BC; Zeng, CK; Tseng, CK
	1998-02-01
发表期刊	SCIENCE IN CHINA SERIES C-LIFE SCIENCES
ISSN	1006-9305
卷号	41 期号:1 页码:9-17
文章类型	Article
摘要	Polysiphonia urceolata R-phycoerythrin and Porphyridium cruentum B-phycoerythrin were degraded with proteinaseK, and then the nearly native gamma subunits were isolated from the reaction mixture. The process of degradation of phycoerythrin with proteinaseK showed that the gamma subunit is located in the central cavity of (alpha beta)(6) hexamer of phycoerythrin. Comparative analysis of the spectra of the native phycoerythrin, the phycoerythrin at pH 12 and the isolated gamma subunit showed that the absorption peaks of phycoerythrobilins on alpha or beta subunit are at 535 nm (or 545 nm) and 565 nm, the fluorescence emission maximum at 580 nm; the absorption peak of phycoerythrobilins on the isolated gamma subunit is at 589 nm, the fluorescence emission peak at 620 nm which overlaps the absorption maximum of C-phycocyanin and perhaps contributes to the energy transfer with high efficiency between phycoerythrin and phycocyanin in phycobilisome; the absorption maximum of phycourobilin on the isolated gamma subunit is at 498 nm, which is the same as that in native phycoerythrin, and the fluorescence emission maximum at 575 nm.; Polysiphonia urceolata R-phycoerythrin and Porphyridium cruentum B-phycoerythrin were degraded with proteinaseK, and then the nearly native gamma subunits were isolated from the reaction mixture. The process of degradation of phycoerythrin with proteinaseK showed that the gamma subunit is located in the central cavity of (alpha beta)(6) hexamer of phycoerythrin. Comparative analysis of the spectra of the native phycoerythrin, the phycoerythrin at pH 12 and the isolated gamma subunit showed that the absorption peaks of phycoerythrobilins on alpha or beta subunit are at 535 nm (or 545 nm) and 565 nm, the fluorescence emission maximum at 580 nm; the absorption peak of phycoerythrobilins on the isolated gamma subunit is at 589 nm, the fluorescence emission peak at 620 nm which overlaps the absorption maximum of C-phycocyanin and perhaps contributes to the energy transfer with high efficiency between phycoerythrin and phycocyanin in phycobilisome; the absorption maximum of phycourobilin on the isolated gamma subunit is at 498 nm, which is the same as that in native phycoerythrin, and the fluorescence emission maximum at 575 nm.
关键词	B-phycoerythrin R-phycoerythrin Gamma Subunit Spectroscopic Properties
学科领域	Biology
收录类别	SCI
语种	英语
WOS记录号	WOS:000072393700002
引用统计	被引频次：14[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/3366
专题	实验海洋生物学重点实验室
作者单位	Acad Sinica, Inst Oceanol, Expt Marine Biol Lab, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Wang, GG,Zhou, BC,Zeng, CK,et al. Isolation, properties and spatial site analysis of gamma subunits of B-phycoerythrin and R-phycoerythrin[J]. SCIENCE IN CHINA SERIES C-LIFE SCIENCES,1998,41(1):9-17.
APA	Wang, GG,Zhou, BC,Zeng, CK,&Tseng, CK.(1998).Isolation, properties and spatial site analysis of gamma subunits of B-phycoerythrin and R-phycoerythrin.SCIENCE IN CHINA SERIES C-LIFE SCIENCES,41(1),9-17.
MLA	Wang, GG,et al."Isolation, properties and spatial site analysis of gamma subunits of B-phycoerythrin and R-phycoerythrin".SCIENCE IN CHINA SERIES C-LIFE SCIENCES 41.1(1998):9-17.

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