A new method of Laminaria japonica strain selection and sporeling raising by the use of gametophyte clones

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	A new method of Laminaria japonica strain selection and sporeling raising by the use of gametophyte clones
	Li, DP; Zhou, ZG; Liu, HH; Wu, CY
	1999-04-01
发表期刊	HYDROBIOLOGIA
ISSN	0018-8158
卷号	399 页码:473-476
文章类型	Article
摘要	One-celled female and male gametophytes of three Laminaria japonica strains were isolated, cultured and gametophyte clones were formed. A technique combining strain selection with sporeling raising by the use of these female and male gametophyte clones was studied. Experiments on 9 different crossing combinations was conducted in November of 1997 in Qingdao, P. R. China. The main economic characteristics, frond length and fresh weight, of sporophytes of different crossing combinations were measured. F-1 sporophytes of No. 2 showed a higher fresh weight and longer length, therefore, No. 2 (Wh860 + x Lid) was selected as a good combination. Its parental female and male gametophyte clones are being mass cultured for sporeling production. By this method, the time needed for strain selection was shortened from 5-6 to 2 years. As compared with the routine method of sporeling raising by the collection of zoospores, the time of sporeling raising of this method decrease by 50%, and the production cost is also reduced by 50%. It is believed that this method will be labour and time saving and a more economic way for strain selection and sporeling raising in L. japonica cultivation industry.; One-celled female and male gametophytes of three Laminaria japonica strains were isolated, cultured and gametophyte clones were formed. A technique combining strain selection with sporeling raising by the use of these female and male gametophyte clones was studied. Experiments on 9 different crossing combinations was conducted in November of 1997 in Qingdao, P. R. China. The main economic characteristics, frond length and fresh weight, of sporophytes of different crossing combinations were measured. F-1 sporophytes of No. 2 showed a higher fresh weight and longer length, therefore, No. 2 (Wh860 + x Lid) was selected as a good combination. Its parental female and male gametophyte clones are being mass cultured for sporeling production. By this method, the time needed for strain selection was shortened from 5-6 to 2 years. As compared with the routine method of sporeling raising by the collection of zoospores, the time of sporeling raising of this method decrease by 50%, and the production cost is also reduced by 50%. It is believed that this method will be labour and time saving and a more economic way for strain selection and sporeling raising in L. japonica cultivation industry.
关键词	Laminaria Japonica Gametophyte Clones Crossing Strain Selection Sporeling Raising
学科领域	Marine & Freshwater Biology
收录类别	SCI ; ISTP
语种	英语
WOS记录号	WOS:000082676900059
引用统计	被引频次：35[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/3354
专题	实验海洋生物学重点实验室
作者单位	Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Li, DP,Zhou, ZG,Liu, HH,et al. A new method of Laminaria japonica strain selection and sporeling raising by the use of gametophyte clones[J]. HYDROBIOLOGIA,1999,399:473-476.
APA	Li, DP,Zhou, ZG,Liu, HH,&Wu, CY.(1999).A new method of Laminaria japonica strain selection and sporeling raising by the use of gametophyte clones.HYDROBIOLOGIA,399,473-476.
MLA	Li, DP,et al."A new method of Laminaria japonica strain selection and sporeling raising by the use of gametophyte clones".HYDROBIOLOGIA 399(1999):473-476.

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