| Genomic DNA Extraction from in vivo Sampled Tissue of Pacific Oyster, Crassostrea gigas | |
| Wang, Xiaotong; Li, Li; Xu, Fei; Zhang, Guofan | |
| 2011 | |
| 发表期刊 | ISRAELI JOURNAL OF AQUACULTURE-BAMIDGEH
 |
| 卷号 | 63 |
| 文章类型 | Article |
| 摘要 | As opposed to other animals, live bivalve shellfish cannot be sampled because of the high mortality associated with opening closed shells. In this paper, we present a new anesthesia method using an MgSO4 seawater solution for sampling live bivalve shellfish. In our experiment, genomic DNA was successfully extracted from mantle tissues of sampled Pacific oyster (Crassostrea gigas) and the DNA was used as a template for polymerase chain reaction (PCR), which indicated that 3 mm(3) of mantle tissue was enough for DNA extraction and subsequent molecular biology testing. The survival rate after the in vivo sampling was high (83.3%), indicating that the sampling method caused only slight harm to the oysters. Thus, MgSO4 anesthesia is a practical method for in vivo sampling of oysters and probably could be applied to other bivalve shellfish species as well, significantly promoting the application of molecular marker-assisted selection in bivalve shellfish breeding and the study of tissue injury and repair mechanisms in bivalve shellfish. |
| 关键词 | Oyster Live Sampling Genomic Dna Mgso4 Anesthesia |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000298952800001 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/32932 |
| 专题 | 海洋生物技术研发中心 |
| 作者单位 | Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Wang, Xiaotong,Li, Li,Xu, Fei,et al. Genomic DNA Extraction from in vivo Sampled Tissue of Pacific Oyster, Crassostrea gigas[J]. ISRAELI JOURNAL OF AQUACULTURE-BAMIDGEH,2011,63. |
| APA | Wang, Xiaotong,Li, Li,Xu, Fei,&Zhang, Guofan.(2011).Genomic DNA Extraction from in vivo Sampled Tissue of Pacific Oyster, Crassostrea gigas.ISRAELI JOURNAL OF AQUACULTURE-BAMIDGEH,63. |
| MLA | Wang, Xiaotong,et al."Genomic DNA Extraction from in vivo Sampled Tissue of Pacific Oyster, Crassostrea gigas".ISRAELI JOURNAL OF AQUACULTURE-BAMIDGEH 63(2011). |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Genomic DNA Extracti（109KB） | 期刊论文 | 出版稿 | 限制开放 | ODC PDDL | 浏览 | |
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