| A practical method of Ludox density gradient centrifugation combined with protargol staining for extracting and estimating ciliates in marine sediments | |
Xu, Kuidong1 ; Du, Yongfen1,2; Lei, Yanli1; Dai, Renhai1,2
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| 2010-11-01 | |
| 发表期刊 | EUROPEAN JOURNAL OF PROTISTOLOGY
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| 卷号 | 46期号:4页码:263-270 |
| 文章类型 | Article |
| 摘要 | Methodological impediments have long been the main problem in estimating the ecological role of marine benthic ciliates. Percoll density centrifugation is currently the most efficient technique for extracting ciliates from fine-grained sediments, while the high cost and low density of Percoll limit its wide application. We developed a protocol of density gradient centrifugation using the cheap sol Ludox HS 40 in combination with the quantitative protargol stain (QPS) to enumerate and identify marine benthic ciliates. The combined Ludox-QPS method involves sample collection and salt reduction, extraction with Ludox centrifugation, and preparation with the QPS technique. The recovery efficiency of Ludox was first tested with azoic sandy and muddy sediments. A 94-100% recovery rate of ciliates was reached. The method was further tested with natural sandy, muddy-sand and muddy sediments. Excellent extraction efficiencies were consistently obtained: an average of 97.6% for ciliates in sand, and 96.9-97.8% for nematodes in the three types of sediments. The high efficiencies indicate that the method allows for simultaneous enumeration of micro- and meiobenthos. Advantages of the new method include: (i) reliable and cost-efficient operation; (ii) appropriate centrifugation for both micro- and meiobenthos; and (iii) applicability to large samples and routine ecological surveys. (C) 2010 Elsevier GmbH. All rights reserved. |
| 关键词 | Protozoa Ludox-qps Method Extraction Efficiency Identification Benthic Ciliates |
| DOI | 10.1016/j.ejop.2010.04.005 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000286498900003 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/32895 |
| 专题 | 海洋生物分类与系统演化实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Xu, Kuidong,Du, Yongfen,Lei, Yanli,et al. A practical method of Ludox density gradient centrifugation combined with protargol staining for extracting and estimating ciliates in marine sediments[J]. EUROPEAN JOURNAL OF PROTISTOLOGY,2010,46(4):263-270. |
| APA | Xu, Kuidong,Du, Yongfen,Lei, Yanli,&Dai, Renhai.(2010).A practical method of Ludox density gradient centrifugation combined with protargol staining for extracting and estimating ciliates in marine sediments.EUROPEAN JOURNAL OF PROTISTOLOGY,46(4),263-270. |
| MLA | Xu, Kuidong,et al."A practical method of Ludox density gradient centrifugation combined with protargol staining for extracting and estimating ciliates in marine sediments".EUROPEAN JOURNAL OF PROTISTOLOGY 46.4(2010):263-270. |
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| A practical method o（422KB） | 期刊论文 | 出版稿 | 限制开放 | ODC PDDL | 浏览 | |
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