Transient expression of lacZ in bombarded unicellular green alga Haematococcus pluvialis

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	Transient expression of lacZ in bombarded unicellular green alga Haematococcus pluvialis
	Teng, CY ; Qin, S ; Liu, JG ; Yu, DZ ; Liang, CW ; Tseng, CK
	2002-12-01
发表期刊	JOURNAL OF APPLIED PHYCOLOGY
ISSN	0921-8971
卷号	14 期号:6 页码:497-500
文章类型	Article
摘要	This paper reports for the first time the transient expression of a reporter gene, LacZ, in the unicellular green alga Haematococcus pluvialis. By employing the micro-particle bombardment method, motile cells in the exponential phase showed transient expression of lacZ. This was detected in bombarded motile cells under the rupture-disc pressures of 3103 KPa and 4137 KPa. Transient expression of LacZ gene could not be observed in non-motile cells of this alga under the same transformation condition. No LacZ background was found in either the motile cells or the non-motile cells. The study suggests a promising potential of the SV40 promoter and the lacZ reporter gene in genetic engineering of unicellular green algae.; This paper reports for the first time the transient expression of a reporter gene, LacZ, in the unicellular green alga Haematococcus pluvialis. By employing the micro-particle bombardment method, motile cells in the exponential phase showed transient expression of lacZ. This was detected in bombarded motile cells under the rupture-disc pressures of 3103 KPa and 4137 KPa. Transient expression of LacZ gene could not be observed in non-motile cells of this alga under the same transformation condition. No LacZ background was found in either the motile cells or the non-motile cells. The study suggests a promising potential of the SV40 promoter and the lacZ reporter gene in genetic engineering of unicellular green algae.
关键词	Green Alga Haematococcus Pluvialis Lacz Micro-particle Bombardment Sv40 Promoter
学科领域	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
收录类别	SCI
语种	英语
WOS记录号	WOS:000181010700008
引用统计	被引频次：55[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/3288
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Teng, CY,Qin, S,Liu, JG,et al. Transient expression of lacZ in bombarded unicellular green alga Haematococcus pluvialis[J]. JOURNAL OF APPLIED PHYCOLOGY,2002,14(6):497-500.
APA	Teng, CY,Qin, S,Liu, JG,Yu, DZ,Liang, CW,&Tseng, CK.(2002).Transient expression of lacZ in bombarded unicellular green alga Haematococcus pluvialis.JOURNAL OF APPLIED PHYCOLOGY,14(6),497-500.
MLA	Teng, CY,et al."Transient expression of lacZ in bombarded unicellular green alga Haematococcus pluvialis".JOURNAL OF APPLIED PHYCOLOGY 14.6(2002):497-500.

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