Institutional Repository of Key Laboratory of Marine Ecology & Environmental Sciences, CAS
| Analysis of metallotionein expression and antioxidant enzyme activities in Meretrix meretrix larvae under sublethal cadmium exposure | |
Wang, Qing1,3; Wang, Xiaomei2; Wang, Xiaoyu1,4; Yang, Hongsheng1 ; Liu, Baozhong2
| |
| 2010-11-15 | |
| 发表期刊 | AQUATIC TOXICOLOGY
 |
| 卷号 | 100期号:4页码:321-328 |
| 文章类型 | Article |
| 摘要 | To investigate the possible role of metallothioneins (MTs) and antioxidant enzymes in cadmium (Cd) tolerance in Meretrix meretrix larvae, a new MT (designated MmMT) gene was identified and cloned from M. meretrix. The full-length cDNA of MmMT consisted of an open reading frame (ORF) of 231 bp encoding a protein of 76 amino acids, with 21 cysteine residues and a conserved structural pattern Cys-x-Cys-x(3)-Cys-Tyr-Gly-x(3)-Cys-x-Cys-x(3)-CYs-x-Cys-Lys at the C-terminus. The deduced amino acid sequence of MmMT showed about 57-84% identity with previously published MT sequences of mussels and oysters. Real-time PCR was used to analyze the expression level of MmMT mRNA at different M. meretrix larval stages under Cd exposure (25 mu g L(-1)). Results showed that Cd could induce the expression of MmMT mRNA in the larvae, and the expression level increased 5.04-fold and 3.99-fold in D-shaped larvae and pediveligers, respectively. Immunolocalization of MmMT in the stressed larvae revealed that MmMT was synthesized in almost all of the soft parts at the trochophore and postlarva stages, whereas it was only synthesized in the velum and epidermis at the D-shaped larva and pediveliger stages. The activities of three antioxidant enzymes, superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx), also were measured in larvae at different developmental stages. Increased enzymatic activities were detected mainly in D-shaped larvae and pediveligers under Cd stress, suggesting that these enzymes respond synchronously with MT. Our results indicate that MmMT and antioxidant enzymes played important roles in counteracting Cd stress in M. meretrix larvae. (C) 2010 Elsevier B.V. All rights reserved. |
| 关键词 | Metallothionein Cadmium Larvae Antioxidant Enzyme Bivalve Meretrix Meretrix |
| DOI | 10.1016/j.aquatox.2010.08.012 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000284787200003 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/32831 |
| 专题 | 海洋生态与环境科学重点实验室 实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 4.Hebei Univ Technol, Minist Educ, Engn Res Ctr Seawater Utilizat Technol, Tianjin 300130, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Wang, Qing,Wang, Xiaomei,Wang, Xiaoyu,et al. Analysis of metallotionein expression and antioxidant enzyme activities in Meretrix meretrix larvae under sublethal cadmium exposure[J]. AQUATIC TOXICOLOGY,2010,100(4):321-328. |
| APA | Wang, Qing,Wang, Xiaomei,Wang, Xiaoyu,Yang, Hongsheng,&Liu, Baozhong.(2010).Analysis of metallotionein expression and antioxidant enzyme activities in Meretrix meretrix larvae under sublethal cadmium exposure.AQUATIC TOXICOLOGY,100(4),321-328. |
| MLA | Wang, Qing,et al."Analysis of metallotionein expression and antioxidant enzyme activities in Meretrix meretrix larvae under sublethal cadmium exposure".AQUATIC TOXICOLOGY 100.4(2010):321-328. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Analysis of metallot（1003KB） | 期刊论文 | 出版稿 | 限制开放 | ODC PDDL | 浏览 | |
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
修改评论